201B6
KUIFMSi004-B
General
Donor Information
General Donor Information |
|
| Sex | female |
| Ethnicity | Caucasian |
Phenotype and Disease related information (Donor) |
|
| Diseases | No disease was diagnosed.
|
Other Genotyping (Donor) |
|
| Is there genome-wide genotyping or functional data available? |
No
|
Donor Relations |
|
| Other cell lines of this donor | |
External Databases (Donor) |
|
| BioSamples | SAMEA7996630 |
Ethics
Also have a look at the ethics information for the parental line
KUIFMSi004-A
.
| Alternatives to consent |
hIPSC Derivation
General |
|
|
The source cell information can be found in the parental cell line
KUIFMSi004-A.
|
|
Reprogramming method |
|
| Vector type | Integrating |
| Vector | Virus (Lentivirus) |
| Genes | |
| Is the used vector excisable? |
Unknown |
| Absence of reprogramming vector(s)? |
Unknown |
| Reprogramming vectors silenced? | |
Vector free reprogramming |
|
| Type of used vector free reprogramming factor(s) |
None
|
Other |
|
| Derived under xeno-free conditions |
Unknown |
| Derived under GMP? |
Unknown |
| Available as clinical grade? |
Unknown |
Culture Conditions
| Surface coating | Matrigel/Geltrex | ||||||
| Feeder cells |
SNL76/7 Cellfinder Ont Id: CELDA_00013400 |
||||||
| Passage method | Mechanically | ||||||
| Medium |
Other medium:
Base medium: Primate ES medium (ReproCELL, Japan)
Main protein source: Serum concentration: 10 % Supplements
|
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| NANOG |
Yes |
|
|
|||
| POU5F1 (OCT-4) |
Yes |
|
|
|||
| SOX2 |
Yes |
|
|
|||
| E-Cadherin |
Yes |
|
||||
| SALL4 |
Yes |
|
||||
| DPPA4 |
Yes |
|
|
|||
| TERT |
Yes |
|
|
|||
| LIN28 |
Yes |
|
||||
| foxd3 |
Yes |
|
||||
| KLF4 |
Yes |
|
|
|||
| c-MYC |
Yes |
|
||||
| DNMT3B |
Yes |
|
||||
| GDF3 |
Yes |
|
||||
| nodal |
Yes |
|
||||
| podxl |
Yes |
|
||||
| LEFTY1 |
Yes |
|
||||
| ZFP42 (REX-1) |
Yes |
|
||||
| FGF4 |
Yes |
|
||||
| Dppa5 |
Yes |
|
||||
| DPPA2 |
Yes |
|
||||
| GABRB3 |
Yes |
|
||||
| TGDF1 |
Yes |
|
||||
| GAL |
Yes |
|
||||
| IFITM1 |
Yes |
|
||||
| UTF1 |
Yes |
|
||||
| GRB7 |
Yes |
|
||||
| CD9 |
Yes |
|
||||
| BRIX1 |
Yes |
|
||||
| MYC |
Yes |
|
||||
| NAT1 |
Yes |
|
Differentiation Potency
In vitro spontaneous differentiation
| Marker | Expressed |
| AFP |
Yes |
| FOXA2 |
Yes |
| Keratin 8 |
Yes |
| Keratin 18 |
Yes |
| SOX17 |
Yes |
In vitro spontaneous differentiation
In vitro directed differentiation
| Marker | Expressed |
| TNNT2 |
Yes |
| MEF2C |
Yes |
| MYL7 |
Yes |
| MYH7B |
Yes |
| NKX2-5 |
Yes |
| alpha-smooth muscle actin |
Yes |
| Desmin |
Yes |
| MSX1 protein |
Yes |
In vitro spontaneous differentiation
In vitro directed differentiation
| Marker | Expressed |
| Tyrosine hydroxylase |
Yes |
| class III beta tubulin |
Yes |
| DDC |
Yes |
| DAT |
Yes |
| CHAT |
Yes |
| LMX1B |
Yes |
| MAP2 |
Yes |
| glial fibrillary acidic protein |
Yes |
| PAX6 |
Yes |
Genotyping
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
46XX
Karyotyping method:
G-Banding
|
Other Genotyping (Cell Line) |
|
Login to share your feedback, experiences or results with the research community.