IPS(FORESKIN)-1
WISCi003-A
General
Cell Line |
|
| hPSCreg name | WISCi003-A |
| Cite as: | WISCi003-A (RRID:CVCL_C430) |
| Alternative name(s) |
IPS(FORESKIN)-1
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| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines | |
| Last update | 30th August 2022 |
| User feedback | |
Provider |
|
| Generator |
University of Wisconsin (WISC)
Contact:
Madison - Thomson Lab |
| Derivation country | United States |
External Databases |
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| BioSamples | SAMEA7784668 |
| Cellosaurus | CVCL_C430 |
| Wikidata | Q54898184 |
General Information |
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| Publications | |
| Projects | |
| * Is the cell line readily obtainable for third parties? |
No |
Donor Information
General Donor Information |
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| Sex | male |
| Age of donor (at collection) | neonate |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
|
| Family history | Not available |
| Is the medical history available upon request? | No |
| Is clinical information available? | No |
Donor Relations |
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| Other cell lines of this donor | |
External Databases (Donor) |
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| BioSamples | SAMEA7783918 |
Ethics
| How may genetic information associated with the cell line be accessed? | |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? |
hIPSC Derivation
General |
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| Source cell type |
Fibroblast from foreskin.
Synonyms
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| Source cell origin |
A retractable double-layered fold of skin and mucous membrane that covers the glans penis and protects the urinary meatus when the penis is not erect.
Synonyms
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| Age of donor (at collection) | neonate |
| Source cell line vendor | ATCC |
Reprogramming method |
|
| Vector type | Integrating |
| Vector | Virus (Lentivirus) |
| Genes | |
| Is the used vector excisable? |
Unknown |
| Absence of reprogramming vector(s)? |
Unknown |
| Reprogramming vectors silenced? | |
Vector free reprogramming |
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| Type of used vector free reprogramming factor(s) |
None
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Other |
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| Selection criteria for clones | Geneticin Selection |
| Derived under xeno-free conditions |
Unknown |
| Derived under GMP? |
Unknown |
| Available as clinical grade? |
Unknown |
Culture Conditions
| Surface coating | Matrigel/Geltrex | ||||||
| Feeder cells |
Mouse Embryonic Fibroblast Cellfinder Ont Id: EFO_0004040 |
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| Medium |
mTeSR™ 2
Supplements
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Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| NANOG |
Yes |
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| POU5F1 (OCT-4) |
Yes |
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| SOX2 |
Yes |
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| SSEA-3 |
Yes |
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| SSEA-4 |
Yes |
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| TRA 1-60 |
Yes |
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| TRA 1-81 |
Yes |
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Transcriptome Characterisation
Differentiation Potency
Genotyping
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
46XY
Passage number: 10
Karyotyping method:
G-Banding
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Other Genotyping (Cell Line) |
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