KUIFMSi011-D

246G5

General

Cell Line

hPSCreg name KUIFMSi011-D
Cite as:
KUIFMSi011-D (RRID:CVCL_C246)
Alternative name(s)
246G5
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
Last update 3rd September 2022
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Provider

Generator Kyoto University (KUIMS)
Derivation country Japan

External Databases

Cellosaurus CVCL_C246
Wikidata Q54583536

General Information

Publications
* Is the cell line readily obtainable for third parties?
No

Donor Information

General Donor Information

Sex male
Age of donor (at collection) neonate

Phenotype and Disease related information (Donor)

Diseases

Donor Relations

Other cell lines of this donor

Ethics

How may genetic information associated with the cell line be accessed?
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell line name BJ
Other names
Source cell type
Synonyms
  • fibroblast
  • Fibroblasts
  • Fibroblast
  • FIBROBLAST
show more synonyms
Source cell origin
Source cell type (free text) ATCC® CRL-2522
Age of donor (at collection) neonate

Reprogramming method

Vector type Integrating
Vector Virus (Lentivirus)
Genes
Is the used vector excisable?
Unknown
Absence of reprogramming vector(s)?
Unknown
Reprogramming vectors silenced?

Vector free reprogramming

Type of used vector free reprogramming factor(s)
None

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells SNL76/7
Cellfinder Ont Id: CELDA_00013400
Passage method Mechanically
Medium Other medium:
Base medium: Primate ES medium (ReproCELL, Japan)
Main protein source:
Serum concentration: %
Supplements
hrbFGF 4 ng/ml %

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
GDF3
Yes
ZFP42 (REX-1)
Yes
POU5F1
Yes
SOX2
Yes
NANOG
Yes
FGF4
Yes
Dppa5
Yes
DPPA4
Yes
DPPA2
Yes
TERT
Yes
MYC
Yes
KLF4
Yes
NAT1
Yes

Genotyping

Karyotyping (Cell Line)

Other Genotyping (Cell Line)