MUNIe004-A-1

General

Cell Line

hPSCreg name MUNIe004-A-1
Cite as:
MUNIe004-A-1
Cell line type Human embryonic stem cell (hESC)
Similar lines
MUNIe004-A
(CCTL 10)
Last update 28th July 2017
User feedback
No feedback available yet.

Login to share your feedback, experiences or results with the research community.

Provider

Generator Masaryk University (MUNI)
Derivation country Czech Republic

External Databases

General Information

* Is the cell line readily obtainable for third parties?
Yes
Subclone of

Donor Information

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases

Ethics

Also have a look at the ethics information for the parental line MUNIe004-A .
Alternatives to consent
Name of accrediting authority involved?
Approval number

hESC Derivation

The source cell information can be found in the parental cell line MUNIe004-A.

Culture Conditions

Feeder cells Mouse Embryonic Fibroblast Cells
Cellfinder Ont Id: EFO_0004040
O2 Concentration 20 %
CO2 Concentration 5 %
Medium Other medium:
Base medium: DMEM/F-12
Main protein source:
Serum concentration: %
Supplements
FGF2 %

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
POU5F1 (OCT-4)
Yes
SSEA-3
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
SSEA-1
No
DNMT3B
Yes
GDF3
Yes
ZFP42 (REX-1)
Yes
GABRB3
Yes
NANOG
Yes
TDGF1
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XX,der(8)t(8;17)(p11.2;q11),del(18)(q21.2q21)[5]/46,XX,del(18)(q21.2q21.3)[10]
Passage number: 158

Other Genotyping (Cell Line)

Genetic Modification