RC-6, RC6

General

Cell Line

hPSCreg name RCe010-A
Cite as:
RCe010-A (RRID:CVCL_C788)
Alternative name(s)
RC-6, RC6
Cell line type Human embryonic stem cell (hESC)
Similar lines
KCLe004-A
(KCL-005_HD1)
Donor diseases:
monogenic disease
RCe009-A
(RC-5, RC5)
Donor diseases:
monogenic disease
SDUe007-A
(KMEB2)
Donor diseases:
monogenic disease
RGIe145-A
(SI-246)
Donor diseases:
monogenic disease
RGIe115-A
(SI-213)
Donor diseases:
monogenic disease
UPSFRe002-A
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Donor diseases:
monogenic disease
Last update 28th November 2017
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Provider

Generator Roslin Cells (RC)
Owner Derivation of Clinical Grade hES Cells
Distributors
Derivation country United Kingdom

External Databases

Cellosaurus CVCL_C788
Wikidata Q54949407

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes

Donor Information

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
The donor is affected.

Ethics

Alternatives to consent are available? Yes
Alternatives to consent
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? HFEA

hESC Derivation

Date of derivation 2008-10-20
Embryo stage Blastula with ICM and Trophoblast
Supernumerary embryos from IVF treatment?
Yes
PGD Embryo?
No
ZP removal technique Mechanical
Cell isolation None
Cell seeding Embryo

Culture Conditions

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
SSEA-1
No
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XY
Passage number: 13

Other Genotyping (Cell Line)