General

Cell Line

hPSCreg name RCe017-A-1
Cite as:
RCe017-A-1
Cell line type Human embryonic stem cell (hESC)
Similar lines
RCe017-A
(RC13, RC-13)
Last update 10th April 2017
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Provider

Generator Roslin Cells (RC)
Owner Derivation of Clinical Grade hES Cells
Distributors
Derivation country United Kingdom

External Databases

General Information

* Is the cell line readily obtainable for third parties?
Yes
Subclone of

Donor Information

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Ethics

Also have a look at the ethics information for the parental line RCe017-A .
Alternatives to consent
Name of accrediting authority involved?
Approval number

hESC Derivation

The source cell information can be found in the parental cell line RCe017-A.

Culture Conditions

Surface coating Matrigel/Geltrex
CO2 Concentration 5 %
Medium StemPro® hESC SFM
Supplements
BSA 25 %
bFGF

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SSEA-4
Yes
TRA 1-60
Yes
SSEA-1
No
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation

Microbiology / Virus Screening

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
47XY, +12
Passage number: 26

Other Genotyping (Cell Line)

Genetic Modification