RC14, RC-14

General

Cell Line

hPSCreg name RCe018-A
Cite as:
RCe018-A (RRID:CVCL_L203)
Alternative name(s)
RC14, RC-14
Cell line type Human embryonic stem cell (hESC)
Similar lines No similar lines found.
Last update 10th April 2017
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Provider

Generator Roslin Cells (RC)
Owner Derivation of Clinical Grade hES Cells
Distributors
Derivation country United Kingdom

External Databases

Cellosaurus CVCL_L203
Wikidata Q54949380

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes

Donor Information

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Ethics

Was the consent voluntarily given? Yes
Alternatives to consent are available? Yes
Alternatives to consent
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Scotland A Research Ethics Committee
Approval number 07/MRE00/56

hESC Derivation

Date of derivation 2010-09-10
Embryo stage Blastula with ICM and Trophoblast
Supernumerary embryos from IVF treatment?
Yes
Separation of research and IVF treatment?
Yes
PGD Embryo?
No
Expansion status 4
Stephenson 2007
ICM morphology A
Stephenson 2007
Trophectoderm morphology g
Stephenson 2007
ZP removal technique Spontaneous Hatching
Cell isolation Trophoectoderm and ICM isolated
Cell seeding Trophectoderm and ICM seeded onto GMP compataible feeder and xeno-free KOSR media combination
Derived under xeno-free conditions?
Yes
Derivation under GMP?
Yes
Available as clinical grade?
Yes

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells dermal fibroblast
Cellfinder Ont Id: CL_0002620
O2 Concentration 5 %
CO2 Concentration 5 %
Medium StemPro® hESC SFM
Supplements
BSA 25 %
bFGF

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SSEA-4
Yes
TRA 1-60
Yes
SSEA-1
No
Morphology pictures
Morphology of colonies of passages 9 and 27
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation

Microbiology / Virus Screening

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
47XY,+8
Passage number: 28

Other Genotyping (Cell Line)