Man-12

General

Cell Line

hPSCreg name CMFTe002-A
Cite as:
CMFTe002-A (RRID:CVCL_L200)
Alternative name(s)
Man-12
Cell line type Human embryonic stem cell (hESC)
Similar lines
UMANe003-A
(Man-11)
Last update 8th February 2021
User feedback
No feedback available yet.

Login to share your feedback, experiences or results with the research community.

Provider

Generator Central Manchester and Manchester Children's University Hospitals NHS (CMFT)
Owner Central Manchester NHS Foundation Trust (CMFT)
Distributors
Derivation country United Kingdom

External Databases

Cellosaurus CVCL_L200
BioSamples SAMEA8172005
Wikidata Q54903988

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Clinical use: allowed
Commercial use: not allowed

Donor Information

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Is the medical history available upon request? Yes
Is clinical information available? Yes

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
Normal Male 46 XY
Karyotyping method: G-Banding

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
Yes
Array CGH

Donor Relations

All cell lines of this donor's relatives
Has sister:

External Databases (Donor)

BioSamples SAMEA4760722

Ethics

Was the embryo established purely for research purposes? No
Have both parents consented to the use of the embryo for ESC derivation? Yes
Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Alternatives to consent are available? Yes
Alternatives to consent
Alternative consent approval number
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. pseudonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent expressly prevent the derivation of pluripotent stem cells? No
How may genetic information associated with the cell line be accessed?
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? HTA, HFEA and UK Stem Cell Bank
Approval number HTA Licence number: 22627 HFEA Licence number: R0171-3-A. UKSCB approval number: SCSC12-27

hESC Derivation

Date of derivation 2011-08-27
Embryo stage Blastula with ICM and Trophoblast
Supernumerary embryos from IVF treatment?
Yes
Separation of research and IVF treatment?
Yes
PGD Embryo?
No
Expansion status 1
Stephenson 2007
ICM morphology A
Stephenson 2007
Trophectoderm morphology a
Stephenson 2007
ZP removal technique Chemical
Cell isolation Mechanical
Cell seeding Isolated ICM
Derived under xeno-free conditions?
No
Derivation under GMP?
Yes
Available as clinical grade?
Yes

Culture Conditions

Surface coating CELLstart
Feeder cells Human Dermal Fibroblast
Cellfinder Ont Id: CELDA_000001419
Passage method Mechanically
O2 Concentration 20 %
CO2 Concentration 5 %
Medium mTeSR™ 2
Supplements
penicillin/streptomycin 1 %
L-glutamine 2 mM
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Unknown
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
Unknown
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Unknown

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
NANOG
Yes
SSEA-4
Yes
TRA 1-60
Yes
POU5F1 (OCT-4)
Yes
SOX2
Yes
SSEA-1
No
Differentiation Potency
ESC Derived Cell Line
Ont Id: EFO_0005738
In vitro spontaneous differentiation
Marker Expressed
GATA Binding Protein 6
Yes
FOXA2
Yes
ESC Derived Cell Line
Ont Id: EFO_0005738
In vitro spontaneous differentiation
Marker Expressed
Actin, Alpha 2, Smooth Muscle, Aorta
Yes
Vimentin
Yes
ESC Derived Cell Line
Ont Id: EFO_0005738
In vitro spontaneous differentiation
Marker Expressed
Tubulin Beta 3 Class III
Yes
Neurofilament, light polypeptide
Yes

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
Normal Male 46 XY
Passage number: p14
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
Array CGH