EM2-7F

General

Cell Line

hPSCreg name PFIZi004-A
Cite as:
PFIZi004-A (RRID:CVCL_9S34)
Alternative name(s)
EM2-7F
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
RCi001-A
(Thp3C-6, RCi88)
Donor's gene variants:
SCN9A, SCN9A
Donor diseases:
Primary erythromelalgia
RCi001-B
(RCi89, Thp3C-9)
Donor's gene variants:
SCN9A, SCN9A
Donor diseases:
Primary erythromelalgia
PFIZi003-A
(EM2-7M)
Donor's gene variants:
SCN9A
Donor diseases:
Primary erythromelalgia
PFIZi001-A
(EM1-3F)
Donor's gene variants:
SCN9A
Donor diseases:
Primary erythromelalgia
PFIZi002-A
(EM1-7M)
Donor's gene variants:
SCN9A
Donor diseases:
Primary erythromelalgia
RCi002-A
(FP5C-14, RCi92)
Donor's gene variants:
SCN9A, SCN9A
Donor diseases:
Primary erythromelalgia
RCi002-B
(FP5C-16, RCi93)
Donor's gene variants:
SCN9A, SCN9A
Donor diseases:
Primary erythromelalgia
STBCi017-A
(SFC811-03-01)
Donor's gene variants:
SCN9A
Donor diseases:
neuropathy
STBCi017-B
(SFC811-03-04)
Donor's gene variants:
SCN9A
Donor diseases:
neuropathy
STBCi017-C
(SFC811-03-05)
Donor's gene variants:
SCN9A
Donor diseases:
neuropathy
STBCi036-A
(SFC814-03-01)
Donor's gene variants:
SCN9A
Donor diseases:
neuropathy
KAIMRCi003-A
(DRVT-iPSC#1)
Donor's gene variants:
SCN9A, CPLX1
Donor diseases:
Dravet Syndrome
STBCi035-A
(SFC813-03-09)
Donor's gene variants:
SCN9A
Donor diseases:
neuropathy
KAIMRCi003-B
(DRVT-iPSC#2)
Donor's gene variants:
SCN9A, CPLX1
Donor diseases:
Dravet Syndrome
STBCi035-B
(SFC813-03-03)
Donor's gene variants:
SCN9A
Donor diseases:
neuropathy
STBCi035-C
(SFC813-03-07)
Donor's gene variants:
SCN9A
Donor diseases:
neuropathy
STBCi129-A
(SFC137-03-01)
Donor's gene variants:
SCN9A
Donor diseases:
neuropathy
Last update 15th July 2019
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Provider

Generator Pfizer Limited - Pfizer (PFIZ)
Derivation country United Kingdom

External Databases

Cellosaurus CVCL_9S34
Wikidata Q54947257

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Subclones

Donor Information

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
The donor is affected.
Synonyms
  • Primary erythermalgia
Genetic variants
SCN9A (target)
2q24.3
Disease associated phenotypes
  • HEAT
  • EXERCISE

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? No
Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Provide contact information of the holder of the original Donor Information Sheet:
Do you (Depositor/Provider) hold the original Donor Consent Form? Yes
Has the donor been informed about how her/his data will be protected? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. pseudonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent expressly prevent the derivation of pluripotent stem cells? No
Does consent pertain to a specific research project? No
Does consent permit unforeseen future research, without further consent? Yes
Does the consent permit uses of donated embryo/tissue or derived cell line intended for clinical treatment or human applications? No
Does consent expressly permit storage of donated embryo/tissue for an unlimited time? No
Does consent prevent the DONATED BIOSAMPLE from being made available to researchers anywhere in the world? Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No

Does consent permit research by

an academic institution? Yes
a public organisation? Yes
a non-profit company? Yes
a for-profit corporation? Yes
Does consent expressly permit collection of genetic information? Yes
Has the donor been informed that their donated biosample or derived cells may be tested for the presence of microbiological agents / pathogens? No
How may genetic information associated with the cell line be accessed?
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Does the consent permit the donor, upon withdrawal of consent, to stop the use of the derived cell line(s) that have already been created from donated samples? No
Does the consent permit the donor, upon withdrawal of consent, to stop delivery or use of information and data about the donor? Yes
Does consent permit access to medical records of the donor? Yes
Please describe how access is provided:
Does consent permit access to any other source of information about the clinical treatment or health of the donor? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved?
Approval number
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? Yes
Name of accrediting authority involved?
Approval number
Are you aware of any further constraints on the use of the donated embryo/tissue or derived cells? No
Is there an MTA available for the cell line? No
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell type
A nucleated precursor of an erythrocyte that lacks hematopoietic lineage markers.
Synonyms
  • normoblast
Source cell origin
A fluid that is composed of blood plasma and erythrocytes.
Synonyms
  • portion of blood
  • vertebrate blood
  • whole blood
Passage number reprogrammed 10

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes

Vector free reprogramming

Other

Selection criteria for clones morphology, pluripotency gene expression, differentiation in to sensory neurons
Derived under xeno-free conditions
Unknown
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzymatically
Dispase
O2 Concentration 21 %
CO2 Concentration 5 %
Medium mTeSR™ 1

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
NANOG
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Neuron
Ont Id: CL_0000540
In vitro directed differentiation

Microbiology / Virus Screening

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46, XY
Passage number: ~10
Karyotyping method: G-Banding

Other Genotyping (Cell Line)