HuES14

General

Cell Line

hPSCreg name HVRDe014-A
Cite as:
HVRDe014-A (RRID:CVCL_B144)
Alternative name(s)
HuES14
Cell line type Human embryonic stem cell (hESC)
Similar lines No similar lines found.
Last update 24th August 2017
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Provider

Generator Harvard University (HVRD)
Contact:
Melton Lab (MLB)
Derivation country United States

External Databases

Cellosaurus CVCL_B144
NIHhESC NIHhESC-09-0027
Wikidata Q54896743

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes

Donor Information

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases

Ethics

Alternatives to consent are available? Yes
Alternatives to consent
Alternative consent approval number NIHhESC-09-0027

hESC Derivation

Embryo stage Blastula with ICM and Trophoblast
Supernumerary embryos from IVF treatment?
Yes
PGD Embryo?
No
Expansion status 4
Stephenson 2007
ICM morphology A
Stephenson 2007
Trophectoderm morphology a
Stephenson 2007
ZP removal technique Chemical
Cell isolation Immunosurgery
Cell seeding Isolated ICM

Culture Conditions

Passage method Enzymatically
CO2 Concentration 5 %
Medium Other medium:
Base medium: Knockout-DMEM
Main protein source: Knock-out serum replacement
Serum concentration: 10 %
Supplements
bFGF %

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
SSEA-3
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
DNMT3B
Yes
GABRB3
Yes
GDF3
Yes
NANOG
Yes
POU5F1 (OCT-4)
Yes
ZFP42 (REX-1)
Yes
TDGF1
Yes
SSEA-1
No
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma
In vitro spontaneous differentiation

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XX
Passage number: 10

Other Genotyping (Cell Line)