MEL-2

General

Cell Line

hPSCreg name SCSe002-A
Cite as:
SCSe002-A (RRID:CVCL_B864)
Alternative name(s)
MEL-2
Cell line type Human embryonic stem cell (hESC)
Similar lines No similar lines found.
Last update 22nd May 2019
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Provider

Generator Stem Cell Sciences plc (SCS)
Derivation country United Kingdom

External Databases

Cellosaurus CVCL_B864
NIHhESC NIHhESC-11-0140
Wikidata Q54952665

General Information

Publications
* Is the cell line readily obtainable for third parties?
No

Donor Information

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases

Ethics

Alternatives to consent
Alternative consent approval number NIHhESC-11-0140

hESC Derivation

Embryo stage Morula
ZP removal technique Enzymatic
Cell isolation Immunosurgery

Culture Conditions

Feeder cells mouse embryonic fibroblast cell
Cellfinder Ont Id: EFO_0004040
CO2 Concentration 5 %
Medium Other medium:
Base medium: High glucose DMEM
Main protein source: Fetal Calf Serum
Serum concentration: %

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
DNMT3B
Yes
GABRB3
Yes
GDF3
Yes
NANOG
Yes
POU5F1 (OCT-4)
Yes
ZFP42 (REX-1)
Yes
SOX2
Yes
TDGF1
Yes
Alkaline Phosphatase
Yes
SSEA-1
No
SSEA-3
Yes
SSEA-4
Yes
TRA 1-81
Yes

Microbiology / Virus Screening

Hepatitis B Negative
Hepatitis C Negative

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XX
Passage number: 5

Other Genotyping (Cell Line)