ESIBIe001-A

HES-1

General

Cell Line

hPSCreg name ESIBIe001-A
Cite as:
ESIBIe001-A (RRID:CVCL_D092)
Alternative name(s)
HES-1
Cell line type Human embryonic stem cell (hESC)
Similar lines No similar lines found.
Last update 13th September 2017
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Provider

Generator ES Cell International Pte Ltd. (ESIBI)
Derivation country Singapore

External Databases

Cellosaurus CVCL_D092
Wikidata Q54882904

General Information

Publications
Projects
* Is the cell line readily obtainable for third parties?
Yes

Donor Information

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases

Ethics

Alternatives to consent are available? Yes
Alternatives to consent

hESC Derivation

Embryo stage Blastula with ICM and Trophoblast
Supernumerary embryos from IVF treatment?
Yes
PGD Embryo?
No
ZP removal technique Enzymatic
Cell isolation Immunosurgery
Cell seeding Isolated ICM

Culture Conditions

Feeder cells mouse embryonic fibroblast cell
Cellfinder Ont Id: EFO_0004040
Passage method Enzymatically and Mechanically
Medium Other medium:
Base medium: High glucose DMEM
Main protein source: Fetal bovine serum
Serum concentration: 20 %
Supplements
FGF 2 %

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
SSEA-4
Yes
TRA 1-60
Yes
DNMT3B
Yes
GABRB3
Yes
GDF3
Yes
NANOG
Yes
POU5F1 (OCT-4)
Yes
ZFP42 (REX-1)
Yes
TDGF1
Yes
SSEA-3
Yes
TRA 1-81
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma
In vitro spontaneous differentiation

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XX
Passage number: 46

Other Genotyping (Cell Line)