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CEBe001-A

Registration Summary:

FC018

CEBe001-A

General

Cell Line
hPSCreg name CEBe001-A
Cite as:
CEBe001-A (RRID:CVCL_B349)
Alternative name(s)
FC018
Cell line type Human embryonic stem cell (hESC)
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Last update 31st August 2022
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Provider
Generator Takara Bio Europe AB (former Cellartis) (CEB)
Derivation country Sweden

External Databases
Cellosaurus CVCL_B349
Wikidata Q54833302

General Information
Publications
* Is the cell line readily obtainable for third parties?
No

Donor Information

General Donor Information
Sex unknown

Phenotype and Disease related information (Donor)
Diseases A disease was diagnosed.
Klinefelter syndrome
The donor is affected.
Synonyms
  • Hypogonadotropic Hypogonadism
  • Klinefelter's syndrome
  • XXY syndrome
  • XXY trisomy
show more synonyms
Disease associated phenotypes
  • Triploid Karyotype

Ethics

Alternatives to consent are available? Yes
Alternatives to consent
Alternative consent approval number
How may genetic information associated with the cell line be accessed?

hESC Derivation

Date of derivation 2001-09-01
Embryo stage Blastula with ICM and Trophoblast
Supernumerary embryos from IVF treatment?
Yes
PGD Embryo?
No
ZP removal technique Enzymatic
Cell isolation Immunosurgery
Cell seeding Isolated ICM

Culture Conditions

Feeder cells mouse embryonic fibroblast cell
Cellfinder Ont Id: EFO_0004040
O2 Concentration 20 %
CO2 Concentration 5 %
Medium Other medium:
Base medium: DMEM/F12 with Glutamax
Main protein source: Anti-Human-Serum (Sigma)
Serum concentration: %
Supplements
Fetal Calf Serum %
hrbFGF %

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
TRA 1-60
Yes
TRA 1-81
Yes
POU5F1 (OCT-4)
Yes
Pluripotency Score Novelty Score Link to microarray data
17.743 1.158 http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-4057/
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma
In vitro spontaneous differentiation

Genotyping

Karyotyping (Cell Line)
Has the cell line karyotype been analysed?
Yes
69XXY
Passage number: 58

Other Genotyping (Cell Line)