KUIMSe002-A

KhES-2

General

Cell Line

hPSCreg name KUIMSe002-A
Cite as:
KUIMSe002-A (RRID:CVCL_B232)
Alternative name(s)
KhES-2
Cell line type Human embryonic stem cell (hESC)
Similar lines No similar lines found.
Last update 3rd September 2018
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Provider

Generator Kyoto University (KUIMS)
Derivation country Japan

External Databases

Cellosaurus CVCL_B232
Wikidata Q54899873

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes

Donor Information

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases

Ethics

Alternatives to consent are available? Yes
Alternatives to consent
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Ethics committee for stem cell research of Ministry of Education, Culture, Sports, Science and Technology (MEXT)

hESC Derivation

Embryo stage Blastula with ICM and Trophoblast
Supernumerary embryos from IVF treatment?
Yes
PGD Embryo?
No
Cell isolation Immunosurgery
Cell seeding Isolated ICM

Culture Conditions

Feeder cells mouse embryonic fibroblast cell
Cellfinder Ont Id: EFO_0004040
Medium Other medium:
Base medium: DMEM/F-12
Main protein source: Knock-out serum replacement
Serum concentration: 20 %
Supplements
2-mercaptoethanol
FGF 2

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
SSEA-3
Yes
SSEA-4
Yes
TRA 1-60
Yes
SSEA-1
No
DNMT3B
Yes
GDF3
Yes
ZFP42 (REX-1)
Yes
GABRB3
Yes
NANOG
Yes
POU5F1 (OCT-4)
Yes
TDGF1
Yes
TRA 1-81
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma
In vitro spontaneous differentiation

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XX
Passage number: 33

Other Genotyping (Cell Line)