MSHRIe002-A

CA2

General

Cell Line

hPSCreg name MSHRIe002-A
Cite as:
MSHRIe002-A (RRID:CVCL_B845)
Alternative name(s)
CA2
Cell line type Human embryonic stem cell (hESC)
Similar lines No similar lines found.
Last update 22nd May 2019
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Provider

Generator Mount Sinai Hospital (MSHRI)
Contact:
Nagy's Lab
Derivation country Canada

External Databases

Cellosaurus CVCL_B845
NIHhESC NIHhESC-11-0138
Wikidata Q54906848

General Information

Publications
* Is the cell line readily obtainable for third parties?
No

Donor Information

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases

Ethics

Alternatives to consent
Alternative consent approval number NIHhESC-11-0138

hESC Derivation

Cell isolation Immunosurgery

Culture Conditions

Feeder cells mouse embryonic fibroblast cell
Cellfinder Ont Id: EFO_0004040
Passage method Enzymatically
Medium Other medium:
Base medium: Knockout-DMEM
Main protein source: Knock-out serum replacement
Serum concentration: 8 %
Supplements
bFGF %

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
DNMT3B
Yes
GABRB3
Yes
GDF3
Yes
NANOG
Yes
POU5F1 (OCT-4)
Yes
ZFP42 (REX-1)
Yes
TDGF1
Yes
SSEA-1
Yes
SSEA-3
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XY

Other Genotyping (Cell Line)