PCCA23-FiPS4F8

General

Cell Line

hPSCreg name UAMi001-A
Cite as:
UAMi001-A (RRID:CVCL_QX83)
Alternative name(s)
PCCA23-FiPS4F8
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
MDCi009-A
(8993-C11)
Donor diseases:
Leigh Disease
TIGETi003-A
(GLD1.3)
Donor diseases:
Krabbe disease
IUFi002-A
Donor diseases:
Leigh syndrome
Last update 2nd August 2022
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Provider

Generator Universidad Autonoma de Madrid (UAM)
Owner Universidad Autonoma de Madrid (UAM)
Derivation country Spain

External Databases

Cellosaurus CVCL_QX83
Wikidata Q54973881
BioSamples SAMEA110403759

General Information

Publications
* Is the cell line readily obtainable for third parties?
No

Donor Information

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Synonyms
  • Propionic Acidemia
Disease associated phenotypes
  • Propionic Acidemia

External Databases (Donor)

BioSamples SAMEA110403917

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Provide contact information of the holder of the original Donor Information Sheet: Eva Richard
Do you (Depositor/Provider) hold the original Donor Consent Form? Yes
Alternatives to consent are available? No
Is there other documentation provided to the donor for consenting purposes? No
Confirm that consent was obtained by a qualified professional Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? Yes
How may genetic information associated with the cell line be accessed? Open Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Universidad Autónoma de Madrid
Approval number CEI 112 - 2189
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell type

Reprogramming method

Vector type Non-integrating
Vector Sendai virus

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Medium mTeSR™ Plus

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
NANOG
Yes
SOX2
Yes
TRA 1-60
Yes
TRA 1-81
Yes
Marker Present Absent
mCpG
OCT4 X
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Marker Expressed
alpha 1-fetoprotein
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro directed differentiation
Marker Expressed
alpha-smooth muscle actin
Yes
Ectoderm
Ont Id: UBERON_0000924
In vitro directed differentiation
Marker Expressed
Tuj1
Yes

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes

Other Genotyping (Cell Line)