ZZU-iPS-DM1-001

General

Cell Line

hPSCreg name ZZUi006-A
Cite as:
ZZUi006-A (RRID:CVCL_VE20)
Alternative name(s)
ZZU-iPS-DM1-001
Cell line type Human induced pluripotent stem cell (hiPSC)
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Donor's gene variants:
DMPK, DMPK
Donor diseases:
myotonic dystrophy type 1
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CBRCULi015-A
(GM23375-1, GM23375 clone 1)
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DMPK – DM1 protein kinase, DMPK – DM1 protein kinase
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Congenital Myotonic Dystrophy
ZZUi015-A
(ZZU-iPS-DM1-002)
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myotonic dystrophy type 1
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myotonic dystrophy type 1
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VUBe003-A
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myotonic dystrophy type 1
VUBe019-A
(VUB19_DM1)
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Steinert myotonic dystrophy
HADe001-A
(HAD 1)
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myotonic dystrophy type 1
KCLe014-A
(KCL018_MD1)
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myotonic dystrophy type 1
CENSOi003-B
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DMD, DMD
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Duchenne muscular dystrophy
CENSOi005-A
(FB76R2c5, CENSOi245)
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CENSOi007-A
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CENSOi001-B
(FB78R2c2, CENSOi249)
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DMD, DMD
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Duchenne muscular dystrophy
CENSOi002-B
(FB74R2c4, CENSOi261)
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DMD, DMD
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Duchenne muscular dystrophy
RCi009-A
(Rci201, FB73c6)
Donor's gene variants:
LRIF1, LRIF1
Donor diseases:
Facioscapulohumeral dystrophy
RCi006-A
(FB69c4, RCi169)
Donor's gene variants:
LRIF1, LRIF1
Donor diseases:
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RCi007-A
(FB70c1, RCi170)
Donor's gene variants:
LRIF1, LRIF1
Donor diseases:
Facioscapulohumeral dystrophy
Last update 29th October 2019
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Provider

Generator The first affiliated hospital (ZZU)
Owner The first affiliated hospital (ZZU)
Derivation country China

External Databases

BioSamples SAMEA104491350
Cellosaurus CVCL_VE20
Wikidata Q54996168

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed

Donor Information

General Donor Information

Sex male
Age of donor (at collection) 45-49
Ethnicity Han

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • Myotonic dystrophy type 1
  • Steinert disease
Genetic variants
DMPK (target)
19q13
Disease associated phenotypes
  • Muscle Weakness
  • Amyotrophy
  • Frontal Alopecia
  • Cataract
  • Dystropy
  • Diabetes Mellitus
  • Heart Defect
  • Shorter Longevity
Family history The patient's son and daughter also suffer from this disease.

Karyotyping (Donor)

Has the donor karyotype been analysed?
No

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA104491351

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? Yes
Do you (Depositor/Provider) hold the original Donor Consent Form? Yes
Alternatives to consent are available? No
Is there other documentation provided to the donor for consenting purposes? No
Confirm that consent was obtained by a qualified professional Yes
Has the donor agreed to be re-contacted? Yes
Has the donor been informed about how her/his data will be protected? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent expressly prevent the derivation of pluripotent stem cells? No
Does consent pertain to a specific research project? No
Does consent permit unforeseen future research, without further consent? Yes
Does the consent permit uses of donated embryo/tissue or derived cell line intended for clinical treatment or human applications? No
Does consent expressly prevent development of commercial products? Yes
Does consent expressly prevent financial gain from any use of the donated embryo/tissue, including any product made from it? Yes
Does consent expressly permit storage of donated embryo/tissue for an unlimited time? Yes
Does consent expressly permit storage of cells derived from the donated embryo/tissue for an unlimited time? Yes
Does consent prevent the DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No

Does consent permit research by

an academic institution? Yes
a public organisation? Yes
a non-profit company? No
a for-profit corporation? No
Does consent expressly permit collection of genetic information? Yes
Does consent expressly permit storage of genetic information? Yes
Does consent prevent dissemination of genetic information? Yes
Has the donor been informed that their donated biosample or derived cells may be tested for the presence of microbiological agents / pathogens? Yes
Has the donor consented to receive information discovered during use of donated embryo/tissue that has significant health implications for the donor? Yes
How may genetic information associated with the cell line be accessed? Controlled Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Does the consent anticipate that the donor will be notified of results or outcomes of any research involving the donated samples or derived cells? No
Does the consent permit the donor, upon withdrawal of consent, to stop the use of the derived cell line(s) that have already been created from donated samples? Yes
Does the consent permit the donor, upon withdrawal of consent, to stop delivery or use of information and data about the donor? Yes
Does consent permit access to medical records of the donor? No
Does consent permit access to any other source of information about the clinical treatment or health of the donor? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Medical Research Ethics Committee of The First Affiliated Hospital of Zhengzhou University
Approval number 2016-007
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? Yes
Name of accrediting authority involved? Medical Research Ethics Committee of The First Affiliated Hospital of Zhengzhou University
Approval number 2016-007
Do you have obligations to third parties in regard to the use of the cell line? Yes
Please describe: School of Life Sciences of Zhengzhou University have obligations in regard to the use of the cell line.
Are you aware of any further constraints on the use of the donated embryo/tissue or derived cells? Yes
Further constraints on use This material and derived cells can't be used for commercial purpose or clinical transplant..
Is there an MTA available for the cell line? No
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? STEMCELL Technologies Inc
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? Yes
Constraints for use or distribution All the resources will be only used for non-commercial scientific research, instead of any other purposes.

hIPSC Derivation

General

Source cell type
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.; These cells may be vimentin-positive, fibronectin-positive, fsp1-positive, MMP-1-positive, collagen I-positive, collagen III-positive, and alpha-SMA-negative.
Source cell origin
Synonyms
  • Left antebrachial region
Age of donor (at collection) 45-49
Collected in 2017
Passage number reprogrammed P3

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Is reprogramming vector detectable?
No
Methods used
PCR
Notes on reprogramming vector detection Reprogramming vector can't be detectable on passage 20.
Files and images showing reprogramming vector expressed or silenced

Vector free reprogramming

Type of used vector free reprogramming factor(s)
None

Other

Selection criteria for clones These colonies will consist of iPSCs, which exhibit a hESC-like morphology characterized by a flatter cobblestone-like appearance with individual cells clearly demarcated from each other in the colonies.
Derived under xeno-free conditions
Yes
Derived under GMP?
Yes
Available as clinical grade?
No

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
Gentle Cell Dissociation Reagent
O2 Concentration 95 %
CO2 Concentration 5 %
Medium mTeSR™ 1
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
No

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
NANOG
Yes
TRA 1-60
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XY
Passage number: P12
Karyotyping method: G-Banding

Other Genotyping (Cell Line)