JNCSRe001-A

BJNhem19

General

Cell Line

hPSCreg name JNCSRe001-A
Cite as:
JNCSRe001-A (RRID:CVCL_B842)
Alternative name(s)
BJNhem19
Cell line type Human embryonic stem cell (hESC)
Similar lines No similar lines found.
Last update 28th July 2017
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Provider

Generator Jawaharlal Nehru Centre for Advanced Scientific Research (JNCSR)
Derivation country India

External Databases

Cellosaurus CVCL_B842
NIHhESC NIHhESC-10-0083
Wikidata Q54797016

General Information

Publications
Projects
* Is the cell line readily obtainable for third parties?
Yes

Donor Information

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases

Ethics

Was the embryo established purely for research purposes? No
Have both parents consented to the use of the embryo for ESC derivation? No
Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Alternatives to consent are available? Yes
Alternatives to consent
Alternative consent approval number NIHhESC-10-0083
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent expressly prevent the derivation of pluripotent stem cells? No
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Institutional Commitee for Stem Cell Reseaarch and Therapy (IC-SCRT)
Approval number

hESC Derivation

Date of derivation 2007-04-01
Embryo stage Blastula with ICM and Trophoblast
Supernumerary embryos from IVF treatment?
Yes
PGD Embryo?
No
Expansion status 2
Stephenson 2007
ICM morphology C
Stephenson 2007
Trophectoderm morphology b
Stephenson 2007
ZP removal technique Spontaneous Hatching
Cell isolation Immunosurgery
Cell seeding Isolated ICM

Culture Conditions

Feeder cells mouse embryonic fibroblast cell
Cellfinder Ont Id: EFO_0004040
Passage method Mechanically
CO2 Concentration 5 %
Medium Other medium:
Base medium: Knockout-DMEM
Main protein source: Fetal Calf Serum
Serum concentration: 5 %
Supplements
L-Glutamine
FGF 2

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
GDF3
Yes
ZFP42 (REX-1)
Yes
GABRB3
Yes
GCT343
Yes
GCTM2
Yes
NANOG
Yes
SOX2
Yes
TDGF1
Yes
THY1/CD90
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation

Microbiology / Virus Screening

HIV 1 Negative
Hepatitis B Negative

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XY
Passage number: 35

Other Genotyping (Cell Line)