OZ

General

Cell Line

hPSCreg name IMHe008-A
Cite as:
IMHe008-A (RRID:CVCL_B022)
Alternative name(s)
OZ
Cell line type Human embryonic stem cell (hESC)
Similar lines
KCLe008-A
(KCL009_trans1)
Donor diseases:
chromosomal disease
IMHe009-A
(OZ-1)
Donor diseases:
chromosomal disease
EDe004-A
(RH 5)
Donor diseases:
Aneuploidy
UPSFRe001-A
(CL01)
Donor diseases:
chromosomal disease
UPSFRe003-A
(CL03)
Donor diseases:
chromosomal disease
Last update 28th July 2017
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Provider

Generator ISTANBUL MEMORIAL HOSPITAL (IMH)
Derivation country Turkey

External Databases

Cellosaurus CVCL_B022
Wikidata Q54897572

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes

Donor Information

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
The donor is affected.
Disease associated phenotypes
  • chromosomal abnormalities

Ethics

Alternatives to consent are available? Yes
Alternatives to consent
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Local Institutional Review Board

hESC Derivation

Date of derivation 2005-04-06
Embryo stage Blastula with ICM and Trophoblast
Supernumerary embryos from IVF treatment?
Yes
PGD Embryo?
No
Expansion status 4
Stephenson 2007
ICM morphology A
Stephenson 2007
Trophectoderm morphology a
Stephenson 2007
ZP removal technique Enzymatic
Cell isolation None
Cell seeding Embryo

Culture Conditions

Surface coating Gelatin
Feeder cells Human Foreskin Fibroblast
Cellfinder Ont Id: CELDA_000001419
Passage method Mechanically
O2 Concentration 21 %
CO2 Concentration 5 %
Medium Other medium:
Base medium: Knockout-DMEM
Main protein source: Fetal bovine serum
Serum concentration: 15 %
Supplements
L-Glutamine
bFGF

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
SSEA-3
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
POU5F1 (OCT-4)
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XX
Passage number: 19

Other Genotyping (Cell Line)