VAL-7

General

Cell Line

hPSCreg name ESe018-A
Cite as:
ESe018-A (RRID:CVCL_9966)
Alternative name(s)
VAL-7
Cell line type Human embryonic stem cell (hESC)
Similar lines No similar lines found.
Last update 28th July 2017
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Provider

Generator Spanish Stem Cell Bank (ES)
Derivation country Spain

External Databases

Cellosaurus CVCL_9966
Wikidata Q54992853

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes

Donor Information

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases

Ethics

Alternatives to consent are available? Yes
Alternatives to consent
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Ethics board of the Prince Felipe Research Centre
Approval number

hESC Derivation

Date of derivation 2007-09-26
Embryo stage Blastula with ICM and Trophoblast
Supernumerary embryos from IVF treatment?
Yes
PGD Embryo?
No
Expansion status 3
Stephenson 2007
ICM morphology B
Stephenson 2007
Trophectoderm morphology a
Stephenson 2007
Cell isolation Laser
Cell seeding Isolated ICM

Culture Conditions

Feeder cells Human foreskin fibroblasts
Cellfinder Ont Id: CELDA_000001419
O2 Concentration 21 %
CO2 Concentration 5 %
Medium Other medium:
Base medium: Knockout-DMEM
Main protein source: Knock-out serum replacement
Serum concentration: 20 %
Supplements
L-Glutamine
hrbFGF

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
NANOG
Yes
POU5F1 (OCT-4)
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
DNMT3B
Yes
GDF3
Yes
ZFP42 (REX-1)
Yes
foxd3
Yes
Amy
No
Cripto
Yes
GABRB3
Yes
Nfh
No
Ren
No
SOX2
Yes
Telomerase Activity
Yes
Thy-1
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma
In vitro spontaneous differentiation

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative

Certificate of Analysis

Is there a certificate of analysis available?
Yes
Passage:

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XY
Passage number: 23

Other Genotyping (Cell Line)