TECHe003-A-1

I6.2

General

Cell Line

hPSCreg name TECHe003-A-1
Cite as:
TECHe003-A-1 (RRID:CVCL_C381)
Alternative name(s)
I6.2
Cell line type Human embryonic stem cell (hESC)
Similar lines
Last update 28th July 2017
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Provider

Generator Technion - Israel Institute of Technology (IIT)
Derivation country Israel

External Databases

Cellosaurus CVCL_C381
Wikidata Q54897065

General Information

* Is the cell line readily obtainable for third parties?
Yes
Subclone of

Donor Information

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases

Ethics

Also have a look at the ethics information for the parental line TECHe003-A .
Alternatives to consent

hESC Derivation

The source cell information can be found in the parental cell line TECHe003-A.

Culture Conditions

Feeder cells Mouse Embryonic Fibroblast Cell
Cellfinder Ont Id: EFO_0004040

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
SSEA-1
No
DNMT3B
Yes
GDF3
Yes
ZFP42 (REX-1)
No
GABRB3
Yes
NANOG
Yes
POU5F1 (OCT-4)
Yes
SOX2
Yes
TDGF1
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XY
Passage number: 30

Other Genotyping (Cell Line)

Genetic Modification