HSF9

General

Cell Line

hPSCreg name SUSMe003-A
Cite as:
SUSMe003-A (RRID:CVCL_D011)
Alternative name(s)
HSF9
Cell line type Human embryonic stem cell (hESC)
Similar lines No similar lines found.
Last update 22nd May 2019
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Provider

Generator Stanford University School of Medicine (SUSM)
Derivation country United States

External Databases

Cellosaurus CVCL_D011
Wikidata Q54896444

General Information

Publications
* Is the cell line readily obtainable for third parties?
No

Donor Information

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases

Ethics

Alternatives to consent

hESC Derivation

Embryo stage Blastula with ICM and Trophoblast
ZP removal technique Mechanical
Cell isolation Mechanical

Culture Conditions

Surface coating Human lung fibroblasts
Passage method Mechanically
Medium Other medium:
Base medium: DMEM
Main protein source: Knock-out serum replacement
Serum concentration: 20 %
Supplements
glutamax
bFGF

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SSEA-1
No
SSEA-3
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma
In vitro spontaneous differentiation

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XX

Other Genotyping (Cell Line)