ESIBIe003-A-2

hES3.2

General

Cell Line

hPSCreg name ESIBIe003-A-2
Cite as:
ESIBIe003-A-2 (RRID:CVCL_7160)
Alternative name(s)
hES3.2
Cell line type Human embryonic stem cell (hESC)
Similar lines
ESIBIe003-A
(HES-3, hES 3)
ESIBIe003-A-11
(KIT+/- ESC)
ESIBIe003-A-8
(KIT D816V ESC 1)
ESIBIe003-A-12
(KIT-/- ESC)
ESIBIe003-A-9
(KIT D816V ESC 2)
ESIBIe003-A-7
(HES-3_44, IRF8-/- ES cell)
Last update 13th September 2017
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Provider

Generator ES Cell International Pte Ltd. (ESIBI)
Derivation country Australia

External Databases

Cellosaurus CVCL_7160
Wikidata Q54882902

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Subclone of

Donor Information

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA104620499

Ethics

Also have a look at the ethics information for the parental line ESIBIe003-A .
Alternatives to consent

hESC Derivation

The source cell information can be found in the parental cell line ESIBIe003-A.

Culture Conditions

Feeder cells hFF (Human Foreskin Fibroblast)
Cellfinder Ont Id: CELDA_000001419
Passage method Enzymatic, Mechanical
CO2 Concentration 5 %
Medium Other medium:
Base medium: Knockout-DMEM
Main protein source: Knock-out serum replacement
Serum concentration: 20 %
Supplements
L-Glutamine
bFGF

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
GATA6
Yes
NANOG
Yes
Alkaline Phosphatase
Yes
POU5F1 (OCT-4)
Yes
SSEA-3
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma
In vitro spontaneous differentiation

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XX

Other Genotyping (Cell Line)

Genetic Modification