T1

General

Cell Line

hPSCreg name KMUe001-A
Cite as:
KMUe001-A (RRID:CVCL_C747)
Alternative name(s)
T1
Cell line type Human embryonic stem cell (hESC)
Similar lines No similar lines found.
Last update 28th July 2017
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Provider

Generator Kaohsiung Medical University (KMU)
Derivation country Taiwan

External Databases

Cellosaurus CVCL_C747
Wikidata Q54900200

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes

Donor Information

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases

Ethics

Alternatives to consent are available? Yes
Alternatives to consent

hESC Derivation

Embryo stage Blastula with ICM and Trophoblast
Supernumerary embryos from IVF treatment?
Yes
ZP removal technique Enzymatic
Cell isolation Mechanical

Culture Conditions

Passage method Enzymatic, Enzymatic, Mechanical
CO2 Concentration 5 %
Medium Other medium:
Base medium: High glucose DMEM
Main protein source: Knock-out serum replacement
Serum concentration: 20 %
Supplements
L-Glutamine
bFGF

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
NANOG
Yes
POU5F1 (OCT-4)
Yes
TERT
Yes
Alkaline Phosphatase
Yes
SSEA-1
No
SSEA-4
Yes
TRA 1-81
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma
In vitro spontaneous differentiation

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XX
Passage number: 56

Other Genotyping (Cell Line)