STR-I-305-APC
INSRMe014-A
General
Cell Line |
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| hPSCreg name | INSRMe014-A |
| Cite as: | INSRMe014-A (RRID:CVCL_C094) |
| Alternative name(s) |
STR-I-305-APC
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| Cell line type | Human embryonic stem cell (hESC) |
| Similar lines |
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| Last update | 3rd September 2022 |
| User feedback | |
Provider |
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| Generator |
INSERM (INSRM)
Contact:
Ontogeny of primordial germ cells |
| Derivation country | France |
External Databases |
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| Cellosaurus | CVCL_C094 |
| Wikidata | Q54970628 |
General Information |
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| * Is the cell line readily obtainable for third parties? |
Yes |
Donor Information
General Donor Information |
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| Sex | male |
Phenotype and Disease related information (Donor) |
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| Diseases | A disease was diagnosed.
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Ethics
| Alternatives to consent are available? | Yes |
| Alternatives to consent | |
| Alternative consent approval number | |
| How may genetic information associated with the cell line be accessed? |
hESC Derivation
| Embryo stage | Blastula with ICM and Trophoblast |
| PGD Embryo? |
Yes |
| ZP removal technique | Enzymatic |
Culture Conditions
| Surface coating | Gelatin | |||||||||
| Passage method | Mechanically | |||||||||
| CO2 Concentration | 5 % | |||||||||
| Medium |
Other medium:
Base medium: Knockout-DMEM
Main protein source: Knock-out serum replacement Serum concentration: 20 % Supplements
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Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| DNMT3B |
Yes |
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| GABRB3 |
Yes |
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| GDF3 |
Yes |
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| POU5F1 (OCT-4) |
Yes |
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| SOX2 |
Yes |
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| TDGF1 |
Yes |
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| SSEA-1 |
No |
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| SSEA-4 |
Yes |
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| TRA 1-81 |
Yes |
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Differentiation Potency
Microbiology / Virus Screening |
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| HIV 1 | Negative |
| HIV 2 | Negative |
| Hepatitis B | Negative |
| Hepatitis C | Negative |
Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
46XY
Passage number: 11
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Other Genotyping (Cell Line) |
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