KCL009_trans1

General

Cell Line

hPSCreg name KCLe008-A
Cite as:
KCLe008-A (RRID:CVCL_A244)
Alternative name(s)
KCL009_trans1
Cell line type Human embryonic stem cell (hESC)
Similar lines
IMHe008-A
(OZ)
Donor diseases:
chromosomal disease
IMHe009-A
(OZ-1)
Donor diseases:
chromosomal disease
EDe004-A
(RH 5)
Donor diseases:
Aneuploidy
UPSFRe001-A
(CL01)
Donor diseases:
chromosomal disease
UPSFRe003-A
(CL03)
Donor diseases:
chromosomal disease
Last update 28th July 2017
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Provider

Generator King's College London (KCL)
Derivation country United Kingdom

External Databases

Cellosaurus CVCL_A244
Wikidata Q54899657

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes

Donor Information

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
The donor is affected.
Disease associated phenotypes
  • chromosomal abnormalities

Ethics

Alternatives to consent are available? Yes
Alternatives to consent

hESC Derivation

PGD Embryo?
Yes

Culture Conditions

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
NANOG
Yes
POU5F1 (OCT-4)
Yes
SSEA-1
No
SSEA-3
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
ZFP42 (REX-1)
Yes
SOX2
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XX,der(7)t(7;12)(q31.1;p13.1)pat
Passage number: 10

Other Genotyping (Cell Line)