Regea 08/023

General

Cell Line

hPSCreg name UTAe007-A
Cite as:
UTAe007-A (RRID:CVCL_B360)
Alternative name(s)
Regea 08/023
Cell line type Human embryonic stem cell (hESC)
Similar lines No similar lines found.
Last update 10th December 2020
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Provider

Generator University of Tampere (UTA)
Derivation country Finland

External Databases

Cellosaurus CVCL_B360
Wikidata Q54949715

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes

Donor Information

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases

Ethics

Alternatives to consent are available? Yes
Alternatives to consent
Alternative consent approval number
How may genetic information associated with the cell line be accessed?
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Regional ethics board, Tampere, Finland
Approval number

hESC Derivation

Date of derivation 2008-02-11
Supernumerary embryos from IVF treatment?
Yes
PGD Embryo?
No
ZP removal technique Spontaneous Hatching
Cell isolation Mechanical
Cell seeding Isolated ICM

Culture Conditions

Feeder cells Human Foreskin Fibroblast
Cellfinder Ont Id: CELDA_000001419
Passage method Mechanically
Medium Other medium:
Base medium: Knockout-DMEM
Main protein source: Knock-out serum replacement
Serum concentration: 20 %
Supplements
glutamax
bFGF

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
NANOG
Yes
POU5F1 (OCT-4)
Yes
SSEA-3
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
SSEA-1
No
ZFP42 (REX-1)
Yes
SOX2
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XY
Passage number: p55

Other Genotyping (Cell Line)