CH-ES2

General

Cell Line

hPSCreg name UBASe001-A
Cite as:
UBASe001-A (RRID:CVCL_9733)
Alternative name(s)
CH-ES2
Cell line type Human embryonic stem cell (hESC)
Similar lines No similar lines found.
Last update 3rd September 2022
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Provider

Generator University of Basel (UBAS)
Derivation country Switzerland

External Databases

Cellosaurus CVCL_9733
Wikidata Q54811847

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes

Donor Information

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases

Ethics

Alternatives to consent are available? Yes
Alternatives to consent
Alternative consent approval number
How may genetic information associated with the cell line be accessed?
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Federal Office of Public Health (OFSP)
Approval number

hESC Derivation

Date of derivation 2008-08-27
Embryo stage Blastula with ICM and Trophoblast
Supernumerary embryos from IVF treatment?
Yes
PGD Embryo?
No
Expansion status 6
Stephenson 2007
ICM morphology A
Stephenson 2007
Trophectoderm morphology b
Stephenson 2007
ZP removal technique Spontaneous Hatching
Cell isolation None
Cell seeding Embryo

Culture Conditions

Feeder cells Human Foreskin Fibroblast
Cellfinder Ont Id: CELDA_000001419
CO2 Concentration 5 %
Medium Other medium:
Base medium: Knockout-DMEM
Main protein source: Knock-out serum replacement
Serum concentration: 20 %
Supplements
glutamax
bFGF

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
POU5F1 (OCT-4)
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
DNMT3B
Yes
GDF3
Yes
nodal
Yes
ZFP42 (REX-1)
Yes
foxd3
Yes
FGF4
Yes
GABRB3
Yes
GAL
Yes
GRB7
Yes
hTERT
Yes
LEFTB
Yes
Lefty-A
Yes
LIN28
Yes
NANOG
Yes
podx1
Yes
SOX2
Yes
TDGF1
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma
In vitro spontaneous differentiation

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XX
Passage number: 60

Other Genotyping (Cell Line)