FES22

General

Cell Line

hPSCreg name UHe002-A
Cite as:
UHe002-A (RRID:CVCL_B133)
Alternative name(s)
FES22
Cell line type Human embryonic stem cell (hESC)
Similar lines No similar lines found.
Last update 16th April 2024
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Provider

Generator University of Helsinki (UH)
Derivation country Finland

External Databases

Cellosaurus CVCL_B133
Wikidata Q54834758

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes

Donor Information

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases

Ethics

Alternatives to consent are available? Yes
Alternatives to consent
Alternative consent approval number
How may genetic information associated with the cell line be accessed?

hESC Derivation

Embryo stage Blastula with ICM and Trophoblast
Supernumerary embryos from IVF treatment?
Yes
PGD Embryo?
No
ZP removal technique Enzymatic
Cell isolation Immunosurgery
Cell seeding Isolated ICM

Culture Conditions

Surface coating MEF
Feeder cells mouse embryonic fibroblast cell
Cellfinder Ont Id: EFO_0004040
O2 Concentration 20 %
CO2 Concentration 5 %
Medium Other medium:
Base medium: Knockout-DMEM
Main protein source: Knock-out serum replacement
Serum concentration: 20 %
Supplements
L-Glutamine %
FGF2 %

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
SSEA-3
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
DNMT3B
Yes
GDF3
Yes
ZFP42 (REX-1)
Yes
FGF4
Yes
GABRB3
Yes
NANOG
Yes
POU5F1 (OCT-4)
Yes
PUM-2
Yes
STELLA
Yes
TDGF1
Yes
TEX-14
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma
In vitro spontaneous differentiation

Microbiology / Virus Screening

HIV 1 Negative
Hepatitis B Negative
Hepatitis C Negative

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XY
Passage number: 13

Other Genotyping (Cell Line)