CHULAe001-A

Chula2.hES

General#

Cell Line

hPSCreg Name CHULAe001-A
Alternative name(s)
Chula2.hES
Cell line type Human embryonic stem cell (hESC)
Last update 28th July 2017
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Provider

Generator Chulalongkorn University (CHULA)
Derivation country Thailand

External Databases

Cellosaurus CVCL_B031

General Information

Publications
Is the cell line available in principle?
Yes

Donor Information#

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases

Ethics#

We are currently revising this section.

hESC Derivation#

Date of derivation 2008-12-12
Embryo stage Blastula with ICM and Trophoblast
Expansion status 2
Stephenson 2007
ICM morphology C
Stephenson 2007
Trophectoderm morphology c
Stephenson 2007
ZP removal technique Enzymatic
Cell isolation None
Cell seeding Embryo

Culture Conditions#

Feeder cells Human Foreskin Fibroblast
Cellfinder Ont Id: CELDA_000001419
CO2 Concentration 5 %
Medium Other medium:
Base medium: Knockout-DMEM
Main protein source: Knock-out serum replacement
Serum concentration: 20 %
Supplements
Non-Essential Amino Acids 1 %
Penicillin-Streptomycin 1 %
Glutamax 1 %
ß-mercaptoethanol 0.1 mM

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
POU5F1 (OCT-4)
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
Telomerase Activity
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma
In vitro spontaneous differentiation

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XY

Other Genotyping (Cell Line)