CW30165

General

Cell Line

hPSCreg name CIRMi858-A
Cite as:
CIRMi858-A (RRID:CVCL_4R68)
Alternative name(s)
CW30165
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
CIRMi885-A
(CW30196)
Donor diseases:
Dilated Cardiomyopathy
CIRMi888-A
(CW30199)
Donor diseases:
Dilated Cardiomyopathy
CIRMi894-A
(CW30207)
Donor diseases:
Dilated Cardiomyopathy
CIRMi896-A
(CW30209)
Donor diseases:
Dilated Cardiomyopathy
CIRMi900-A
(CW30213)
Donor diseases:
Dilated Cardiomyopathy
CIRMi903-A
(CW30216)
Donor diseases:
Dilated Cardiomyopathy
CIRMi904-A
(CW30219)
Donor diseases:
Dilated Cardiomyopathy
CIRMi908-A
(CW30223)
Donor diseases:
Dilated Cardiomyopathy
CIRMi912-A
(CW30227)
Donor diseases:
Dilated Cardiomyopathy
CIRMi915-A
(CW30230)
Donor diseases:
Dilated Cardiomyopathy
CIRMi924-A
(CW30240)
Donor diseases:
Dilated Cardiomyopathy
CIRMi926-A
(CW30242)
Donor diseases:
Dilated Cardiomyopathy
CIRMi928-A
(CW30244)
Donor diseases:
Dilated Cardiomyopathy
CIRMi931-A
(CW30247)
Donor diseases:
Dilated Cardiomyopathy
CIRMi932-A
(CW30248)
Donor diseases:
Dilated Cardiomyopathy
CIRMi936-A
(CW30254)
Donor diseases:
Dilated Cardiomyopathy
CIRMi940-A
(CW30259)
Donor diseases:
Dilated Cardiomyopathy
CIRMi941-A
(CW30260)
Donor diseases:
Dilated Cardiomyopathy
CIRMi944-A
(CW30263)
Donor diseases:
Dilated Cardiomyopathy
CIRMi946-A
(CW30265)
Donor diseases:
Dilated Cardiomyopathy
CIRMi948-A
(CW30267)
Donor diseases:
Dilated Cardiomyopathy
CIRMi949-A
(CW30268)
Donor diseases:
Dilated Cardiomyopathy
CIRMi955-A
(CW30275)
Donor diseases:
Dilated Cardiomyopathy
CIRMi967-A
(CW30290)
Donor diseases:
Dilated Cardiomyopathy
CIRMi974-A
(CW30299)
Donor diseases:
Dilated Cardiomyopathy
CIRMi976-A
(CW30302)
Donor diseases:
Dilated Cardiomyopathy
CIRMi978-A
(CW30305)
Donor diseases:
Dilated Cardiomyopathy
CIRMi979-A
(CW30306)
Donor diseases:
Dilated Cardiomyopathy
CIRMi981-A
(CW30309)
Donor diseases:
Dilated Cardiomyopathy
CIRMi987-A
(CW30317)
Donor diseases:
Dilated Cardiomyopathy
CIRMi990-A
(CW30320)
Donor diseases:
Dilated Cardiomyopathy
CIRMi992-A
(CW30322)
Donor diseases:
Dilated Cardiomyopathy
CIRMi995-A
(CW30325)
Donor diseases:
Dilated Cardiomyopathy
CIRMi996-A
(CW30326)
Donor diseases:
Dilated Cardiomyopathy
CIRMi00D-A
(CW30334)
Donor diseases:
Dilated Cardiomyopathy
CIRMi00I-A
(CW30339)
Donor diseases:
Dilated Cardiomyopathy
CIRMi00L-A
(CW30343)
Donor diseases:
Dilated Cardiomyopathy
CIRMi00Q-A
(CW30349)
Donor diseases:
Dilated Cardiomyopathy
CIRMi00R-A
(CW30350)
Donor diseases:
Dilated Cardiomyopathy
CIRMi01A-A
(CW30361)
Donor diseases:
Dilated Cardiomyopathy
CIRMi01E-A
(CW30369)
Donor diseases:
Dilated Cardiomyopathy
CIRMi01I-A
(CW30376)
Donor diseases:
Dilated Cardiomyopathy
CIRMi01J-A
(CW30380)
Donor diseases:
Dilated Cardiomyopathy
CIRMi01N-A
(CW30387)
Donor diseases:
Dilated Cardiomyopathy
CIRMi01R-A
(CW30392)
Donor diseases:
Dilated Cardiomyopathy
CIRMi02B-A
(CW30404)
Donor diseases:
Dilated Cardiomyopathy
CIRMi02J-A
(CW30415)
Donor diseases:
Dilated Cardiomyopathy
CIRMi02L-A
(CW30418)
Donor diseases:
Dilated Cardiomyopathy
CIRMi02M-A
(CW30419)
Donor diseases:
Dilated Cardiomyopathy
CIRMi02O-A
(CW30421)
Donor diseases:
Dilated Cardiomyopathy
Last update 26th September 2023
User feedback
No feedback available yet.

Login to share your feedback, experiences or results with the research community.

Provider

Generator California Institute for Regenerative Medicine (CIRM)
Owner California Institute for Regenerative Medicine (CIRM)
Distributors
Derivation country United States

External Databases

BioSamples SAMEA114427118
Cellosaurus CVCL_4R68
Wikidata Q54815505

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: allowed

Donor Information

General Donor Information

Sex female
Age of donor (at collection) 50-54

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
DILATED CARDIOMYOPATHY
Synonyms
  • Congestive Cardiomyopathy
  • Dilated Cardiomyopathy

External Databases (Donor)

BioSamples SAMEA114427119

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Please provide contact information of the holder of the original Donor Information Sheet. info@cirm.ca.gov
Do you (Depositor/Provider) hold the original Donor Consent Form? No
If you do not hold the Donor Consent Form, do you know who does? Yes
Please provide the contact information info@cirm.ca.gov
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. pseudonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
How may genetic information associated with the cell line be accessed? Controlled Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Stanford University
Approval number eprotocol 27522
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? Yes
Name of accrediting authority involved? Stanford University
Approval number eprotocol 27522
Is there an MTA available for the cell line? Yes

hIPSC Derivation

General

Source cell type
A cell found predominately in the blood.
Age of donor (at collection) 50-54

Reprogramming method

Vector type Non-integrating
Vector Episomal
Is reprogramming vector detectable?
No

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Medium Essential 8™

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
NANOG
Yes
Lin28
Yes
Undifferentiated cells were analyzed by proprietary assays approved by California Institute of Regenerative Medicine (CIRM) as described in the attached publication. Pluripotency of established iPSC cultures were tested by qPCR on bulk samples using 48 genes including standard pluripotency genes (listed above). Samples were assigned a passing score after analysis with a non-supervised classifier based on an appropriate sample set. (Novak, T. 2015. Drug Discovery World pp. 47-53.)
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
Mesoderm
Ont Id: UBERON_0000926
Ectoderm
Ont Id: UBERON_0000924

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
Normal

Other Genotyping (Cell Line)