ReliCellhES1
DALCe001-A
General#
Cell Line |
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| hPSCreg Name | DALCe001-A |
| Alternative name(s) |
ReliCellhES1
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| Cell line type | Human embryonic stem cell (hESC) |
| Last update | 28th July 2017 |
| User feedback | |
Provider |
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| Generator |
Dhirubhai Ambani Life Sciences Center (DALC)
Contact:
Embryonic stem cell group |
| Derivation country | India |
External Databases |
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| Cellosaurus | CVCL_B362 |
General Information |
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| Publications |
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| Is the cell line available in principle? |
No |
Donor Information#
General Donor Information |
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| Sex | female |
Phenotype and Disease related information (Donor) |
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| Diseases | |
Ethics#
| Alternatives to consent are available? | Yes |
| Alternatives to consent |
hESC Derivation#
| Embryo stage | Blastula with ICM and Trophoblast |
| PGD Embryo? |
No |
| ZP removal technique | Enzymatic |
| Cell isolation | Immunosurgery |
Culture Conditions#
| Feeder cells |
Mouse Embryonic Fibroblast Cellfinder Ont Id: EFO_0004040 |
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| Passage method | Mechanically | |||||||||
| Medium |
Other medium:
Base medium: DMEM/F-12
Main protein source: Fetal bovine serum Serum concentration: 15 % Supplements
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Characterisation#
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
| NANOG |
Yes |
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| POU5F1 (OCT-4) |
Yes |
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| ZFP42 (REX-1) |
Yes |
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| SOX2 |
Yes |
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| Alkaline Phosphatase |
Yes |
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| SSEA-3 |
Yes |
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| SSEA-4 |
Yes |
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| TRA 1-60 |
Yes |
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| TRA 1-81 |
Yes |
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Differentiation Potency
Genotyping#
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
46XX
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Other Genotyping (Cell Line) |
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