VAL-7

General#

Cell Line

hPSCreg Name ESe018-A
Alternative name(s)
VAL-7
Cell line type Human embryonic stem cell (hESC)
Last update 28th July 2017
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Provider

Generator Spanish Stem Cell Bank (ES)
Derivation country Spain

External Databases

Cellosaurus CVCL_9966

General Information

Publications
Is the cell line available in principle?
Yes

Donor Information#

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases

Ethics#

We are currently revising this section.

hESC Derivation#

Date of derivation 2007-09-26
Embryo stage Blastula with ICM and Trophoblast
Supernumerary embryos from IVF treatment?
Yes
PGD Embryo?
No
Expansion status 3
Stephenson 2007
ICM morphology B
Stephenson 2007
Trophectoderm morphology a
Stephenson 2007
Cell isolation Laser
Cell seeding Isolated ICM

Culture Conditions#

Feeder cells Human foreskin fibroblasts
Cellfinder Ont Id: CELDA_000001419
O2 Concentration 21 %
CO2 Concentration 5 %
Medium Other medium:
Base medium: Knockout-DMEM
Main protein source: Knock-out serum replacement
Serum concentration: 20 %
Supplements
L-Glutamine
hrbFGF

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG
Yes
POU5F1 (OCT-4)
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
DNMT3B
Yes
GDF3
Yes
ZFP42 (REX-1)
Yes
foxd3
Yes
Amy
No
Cripto
Yes
GABRB3
Yes
Nfh
No
Ren
No
SOX2
Yes
Telomerase Activity
Yes
Thy-1
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma
In vitro spontaneous differentiation

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative

Certificate of Analysis

Is there a certificate of analysis available?
Yes
Passage:

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XY

Other Genotyping (Cell Line)