hES-8
HUSTe001-A
General#
Cell Line |
|
| hPSCreg Name | HUSTe001-A |
| Alternative name(s) |
hES-8
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| Cell line type | Human embryonic stem cell (hESC) |
| Last update | 22nd May 2019 |
| User feedback | |
Provider |
|
| Generator |
Huazhong University of Science and Technology (HUST)
Contact:
Reproductive Medicine Center |
| Derivation country | China |
External Databases |
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| Cellosaurus | CVCL_C117 |
General Information |
|
| * Is the cell line readily obtainable for third parties? |
No |
Donor Information#
General Donor Information |
|
| Sex | female |
Phenotype and Disease related information (Donor) |
|
| Diseases | |
Ethics#
| Alternatives to consent are available? | Yes |
| Alternatives to consent |
hESC Derivation#
| Embryo stage | Blastula with ICM and Trophoblast |
| Supernumerary embryos from IVF treatment? |
No
Other hESC source:
IVF
|
| PGD Embryo? |
No |
| ZP removal technique | Enzymatic |
| Cell isolation | Immunosurgery |
| Cell seeding | Isolated ICM |
Culture Conditions#
| Feeder cells |
mouse embryonic fibroblast cells Cellfinder Ont Id: EFO_0004040 |
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| Passage method | Mechanically | |||||||||
| Medium |
Other medium:
Base medium: DMEM
Main protein source: Fetal bovine serum Serum concentration: 20 % Supplements
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Characterisation#
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
| POU5F1 (OCT-4) |
Yes |
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| Alkaline Phosphatase |
Yes |
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| SSEA-1 |
No |
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| SSEA-4 |
Yes |
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| TRA 1-60 |
Yes |
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| TRA 1-81 |
Yes |
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Differentiation Potency
Genotyping#
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
46XX
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Other Genotyping (Cell Line) |
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