KMUGMCi006-B

TS271 #5 MT

General

Cell Line

hPSCreg name KMUGMCi006-B
Cite as:
KMUGMCi006-B (RRID:CVCL_D0H8)
Alternative name(s)
TS271 #5 MT
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
KMUGMCi006-C
(TS271 #21 WT)
Donor diseases:
Tuberous Sclerosis
KMUGMCi006-D
(TS271 #23 WT)
Donor diseases:
Tuberous Sclerosis
KMUGMCi006-E
(TS271 #4 MT)
Donor diseases:
Tuberous Sclerosis
KMUGMCi006-F
(TS271 #20 MT)
Donor diseases:
Tuberous Sclerosis
KMUGMCi006-A
(TS271 #12 WT)
Donor diseases:
Tuberous Sclerosis
PFIZi032-A
(B214c8)
Donor's gene variants:
TSC2
Donor diseases:
Tuberous Sclerosis
EMCi169-B
(Clone_3)
Donor diseases:
Tuberous sclerosis complex
EMCi169-C
(Clone_9)
Donor diseases:
Tuberous sclerosis complex
EMCi169-D
(Clone_4)
Donor diseases:
Tuberous sclerosis complex
EMCi169-E
(Clone_11)
Donor diseases:
Tuberous sclerosis complex
EMCi169-A
(Clone 1)
Donor diseases:
Tuberous sclerosis complex
HEBHMUi012-A
Donor diseases:
Hypertension
BBANTWi010-A
(iPSC_PBMC_LDS_TGFB3_KE2M_C9)
Donor diseases:
Loeys-Dietz syndrome 5
SHEHi002-A
(iPS36)
Donor's gene variants:
CACNA1C
Donor diseases:
Long QT Syndrome 8
PCIi027-A
(PC086)
Donor diseases:
Waardenburg syndrome
INSRMi019-A
(PC129K8)
Donor diseases:
myofibrillar myopathy 1
INSRMi020-A
(PC130k2c)
Donor diseases:
myofibrillar myopathy 1
INSRMi012-C
(PC173T19)
Donor diseases:
myofibrillar myopathy 1
INSRMi013-A
(PC179c1)
Donor diseases:
myofibrillar myopathy 1
BCHNi001-A
(BCHi001-A)
Donor diseases:
Bardet-Biedl Syndrome
UOMi009-A
(ACS-hiPSC-HPP3)
Donor diseases:
Hypophosphatasia
CUIMCi005-A
(BB#2, BB9068#2)
Donor diseases:
Stargardt Disease
SCTCi014-A
(IPS17-00056)
Donor diseases:
Age-Related Macular Degeneration
MUBi002-A
(CF 001)
Donor diseases:
Cystic fibrosis
USPi001-A
(iPS_LGH_AHDC1_P1)
Donor's gene variants:
AHDC1
Donor diseases:
Xia-Gibbs Syndrome
USPi002-A
(iPS_LGH_AHDC1_P2)
Donor's gene variants:
AHDC1
Donor diseases:
Xia-Gibbs Syndrome
USPi003-A
(iPS_LGH_AHDC1_P3)
Donor's gene variants:
AHDC1
Donor diseases:
Xia-Gibbs Syndrome
SCTCi012-A
(IPS19-00027)
Donor diseases:
age-related macular degeneration
UOMi008-A
(ACS-hiPSC-HPP2)
Donor diseases:
Hypophosphatasia
SCTCi013-A
(IPS19-00053)
Donor diseases:
age-related macular degeneration
IAIi001-A
(IAIi001RSTS2-65-A)
Donor diseases:
Rubinstein-Taybi syndrome
IMAGINi022-A
(IMAGINE022)
Donor diseases:
Waardenburg syndrome
PCIi026-A
(PC085)
Donor diseases:
Waardenburg syndrome
IMBAi017-A
(B002-ARID1B#8)
Donor diseases:
Coffin-Siris Syndrome
PCIi025-A
(PC084)
Donor diseases:
Waardenburg syndrome
PCIi032-A
(PC087)
Donor diseases:
Waardenburg Syndrome
USFi001-A
(1CN1.5)
Donor diseases:
Friedreich Ataxia
Last update 16th March 2023
User feedback
No feedback available yet.

Login to share your feedback, experiences or results with the research community.

Provider

Generator Kanazawa Medical University (KMUGMC)

External Databases

BioSamples SAMEA112774597
Cellosaurus CVCL_D0H8
Wikidata Q123032822

General Information

Publications
* Is the cell line readily obtainable for third parties?
No

Donor Information

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • Tuberous Sclerosis Complex
  • TSC
  • Tuberous Sclerosis
  • Tuberous sclerosis
  • Tuberous Sclerosis Syndrome
  • tuberous sclerosis
  • Bourneville's Disease
show more synonyms

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA112750648

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? Yes
Do you (Depositor/Provider) hold the original Donor Consent Form? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
How may genetic information associated with the cell line be accessed? Open Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved?
Approval number
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell type
A peripheral blood cell with a single nucleus. This category includes lymphocytes and monocytes.
Synonyms
  • PERIPHERAL BLOOD MONONUCLEAR CELL
  • Peripheral Blood Mononuclear Cell
  • PBMC
Source cell origin
A peripheral blood cell with a single nucleus. This category includes lymphocytes and monocytes.
Synonyms
  • PERIPHERAL BLOOD MONONUCLEAR CELL
  • Peripheral Blood Mononuclear Cell
  • PBMC

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Is reprogramming vector detectable?
No
Methods used
RT-PCR

Vector free reprogramming

Other

Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions

Surface coating iMatrix
Feeder cells
No
Passage method Enzymatically
TrypLE
Medium Other medium:
Base medium: StemFit
Main protein source:
Serum concentration: %
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
No

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
ESRRB
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Marker Expressed
FOXA2
Yes
SOX17
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro directed differentiation
Marker Expressed
TBXT
Yes
TBX6
Yes
Ectoderm
Ont Id: UBERON_0000924
In vitro directed differentiation
Marker Expressed
PAX6
Yes
OTX2
Yes

Microbiology / Virus Screening

Mycoplasma Negative

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
figure of virtual karyotype
Karyotyping method: G-Banding

Other Genotyping (Cell Line)