243H7
KUIFMSi009-B
General
Cell Line |
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| hPSCreg name | KUIFMSi009-B |
| Cite as: | KUIFMSi009-B (RRID:CVCL_C103) |
| Alternative name(s) |
243H7
|
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines |
KUIFMSi009-A (243H1) |
| Last update | 17th October 2022 |
| User feedback | |
Provider |
|
| Generator |
Kyoto University (KUIMS)
Contact:
iPS Cell Research and Application |
| Derivation country | Japan |
External Databases |
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| Cellosaurus | CVCL_C103 |
| Wikidata | Q54583513 |
General Information |
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| Publications | |
| * Is the cell line readily obtainable for third parties? |
No |
Donor Information
General Donor Information |
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| Sex | male |
| Age of donor (at collection) | 65-69 |
Phenotype and Disease related information (Donor) |
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| Diseases | |
Donor Relations |
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| Other cell lines of this donor | |
Ethics
| How may genetic information associated with the cell line be accessed? | |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? |
hIPSC Derivation
General |
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| Source cell type |
Synonyms
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| Source cell type (free text) | primary human fibroblast-like synoviocytes (HFLS) |
| Age of donor (at collection) | 65-69 |
Reprogramming method |
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| Vector type | Integrating |
| Vector | Virus (Lentivirus) |
| Genes | |
| Is the used vector excisable? |
Unknown |
| Absence of reprogramming vector(s)? |
Unknown |
| Reprogramming vectors silenced? | |
Vector free reprogramming |
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| Type of used vector free reprogramming factor(s) |
None
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Other |
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| Derived under xeno-free conditions |
Unknown |
| Derived under GMP? |
Unknown |
| Available as clinical grade? |
Unknown |
Culture Conditions
| Surface coating | Matrigel/Geltrex | ||||||
| Feeder cells |
SNL76/7 Cellfinder Ont Id: CELDA_00013400 |
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| Passage method | Mechanically | ||||||
| Medium |
Other medium:
Base medium: Primate ES medium ( ReproCELL, Japan)
Main protein source: Serum concentration: % Supplements
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Characterisation
Analysis of Undifferentiated Cells
Differentiation Potency
In vitro spontaneous differentiation
| Marker | Expressed |
| AFP |
Yes |
| FOXA2 |
Yes |
| Keratin 8 |
Yes |
| Keratin 18 |
Yes |
| SOX17 |
Yes |
In vitro spontaneous differentiation
| Marker | Expressed |
| Actin, alpha 2, smooth muscle |
Yes |
| Desmin |
Yes |
| MSX1 protein |
Yes |
In vitro spontaneous differentiation
| Marker | Expressed |
| class III beta tubulin |
Yes |
| glial fibrillary acidic protein |
Yes |
| MAP2 |
Yes |
| PAX6 |
Yes |
Genotyping
Karyotyping (Cell Line) |
|
Other Genotyping (Cell Line) |
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