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WAe001-A-3U

Registration Summary:

H1_XO_1

The cell line is not validated yet.
WAe001-A-3U

General

iPSC Line
hPSCreg name WAe001-A-3U
Cite as:
WAe001-A-3U
Alternative name(s)
H1_XO_1
iPSC line type Human embryonic stem cell (hESC)
Similar iPSC lines No similar lines found.
Last update 12th August 2026
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Provider
Generator The Francis Crick Institute Limited (CRICK)

External iPSC Databases
BioSamples SAMEA123340336

General iPSC Information
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
Subclone of

iPSC Genetic Modification

Disease/phenotype related modifications
Turner syndrome
45, X MONOSOMY
Synonyms
  • gonadal dysgenesis
  • monosomy X
  • 45, X syndrome
  • 45,X gonadal dysgenesis
  • 45,X syndrome
  • 45,X/46,XX syndrome
  • 45,X0 syndrome
  • 45X syndrome
  • Schereshevkii Turner syndrome
  • Turner Varny syndrome
  • Ullrich-Turner syndrome
  • chromosome X monosomy X
  • genital dwarfism
  • genital dwarfism, Turner type
  • gonadal dysgenesis (45,X)
  • gonadal dysgenesis Turner type
  • karyotype 45, X
show more synonyms
Genetic modifications
Isogenic modification
entire Y chromosome loss
CRISPR/Cas9 system was used on isolated male (XY) iPSCs to generate XO cell lines. Single guide RNA was used to shred the Y chromosome centromere.

Donor Information

General Donor Information
Sex male
Ethnicity N/A

Phenotype and Disease related information (Donor)
Diseases No disease was diagnosed.
Family history NO
Is the medical history available upon request? NO
Is clinical information available? NO

Other Genotyping (Donor)
Is there genome-wide genotyping or functional data available?
No

Donor Relations
Other cell lines of this donor
All cell lines of this donor's relatives

External Databases (Donor)
BioSamples SAMEA7767418

Ethics

Also have a look at the ethics information for the parental line WAe001-A .
Is there an MTA available for the cell line? No
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? No

hESC Derivation

The source cell information can be found in the parental cell line WAe001-A.

hPSC Culture Conditions

Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
versene
O2 Concentration 21 %
CO2 Concentration 5 %
Medium mTeSR™ Plus
Has Rock inhibitor (Y27632) been used at passage previously with this iPSC line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this iPSC line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this iPSC line?
Yes

hPSC Characterisation

Analysis of undifferentiated iPSCs
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
SOX2
Yes
POU5F1 (OCT-4)
Yes
NANOG
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Marker Expressed
GATA6
Yes
SOX17
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro directed differentiation
Marker Expressed
TBXT
Yes
NKX2.5
Yes
Ectoderm
Ont Id: UBERON_0000924
In vitro directed differentiation
Marker Expressed
PAX6
Yes
OTX2
Yes

Genotyping

Karyotyping (iPSC Line)
Has the iPSC line karyotype been analysed?
Yes
45, XO
H1_XO1_LP-WGS.png
Karyotyping method: Low pass Whole Genome Sequencing

Other Genotyping (iPSC Line)