WAe001-A-3X

H1_UTY_KO

The cell line is not validated yet.

General

iPSC Line

hPSCreg name WAe001-A-3X
Cite as:
WAe001-A-3X
Alternative name(s)
H1_UTY_KO
iPSC line type Human embryonic stem cell (hESC)
Similar iPSC lines No similar lines found.
Last update 12th August 2026
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Provider

Generator The Francis Crick Institute Limited (CRICK)

External iPSC Databases

BioSamples SAMEA123340339

General iPSC Information

* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
Subclone of

iPSC Genetic Modification

Genetic modifications not related to a disease
Gene knock-out
Yq11.221
CRISPR/Cas9 system with two RNA guides were used on isolated male (XY) iPSCs to generate UTY knock-out cell line.
CRISPR-associated (CRISPR/Cas) System

Donor Information

General Donor Information

Sex male
Ethnicity N/A

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Family history NO
Is the medical history available upon request? NO
Is clinical information available? NO

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor
All cell lines of this donor's relatives

External Databases (Donor)

BioSamples SAMEA7767418

Ethics

Also have a look at the ethics information for the parental line WAe001-A .
Is there an MTA available for the cell line? No
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? No

hESC Derivation

The source cell information can be found in the parental cell line WAe001-A.

hPSC Culture Conditions

Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
versene
O2 Concentration 21 %
CO2 Concentration 5 %
Medium mTeSR™ Plus

hPSC Characterisation

Analysis of undifferentiated iPSCs
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
SOX2
Yes
POU5F1 (OCT-4)
Yes
NANOG
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Marker Expressed
GATA6
Yes
SOX17
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro directed differentiation
Marker Expressed
TBXT
Yes
NKX2.5
Yes
Ectoderm
Ont Id: UBERON_0000924
In vitro directed differentiation
Marker Expressed
PAX6
Yes
OTX2
Yes

Genotyping

Karyotyping (iPSC Line)

Has the iPSC line karyotype been analysed?
Yes
46, XY
Karyotyping method: Low pass Whole Genome Sequencing

Other Genotyping (iPSC Line)