WA15
WISCe001-A
General
iPSC Line |
|
| hPSCreg name | WISCe001-A |
| Cite as: | WISCe001-A (RRID:CVCL_9776) |
| Alternative name(s) |
WA15
|
| iPSC line type | Human embryonic stem cell (hESC) |
| Similar iPSC lines | |
| Last update | 22nd May 2019 |
| User feedback | |
Provider |
|
| Generator |
University of Wisconsin (WISC)
Contact:
Madison - Thomson Lab |
| Derivation country | United States |
External iPSC Databases |
|
| Cellosaurus | CVCL_9776 |
| NIHhESC | NIHhESC-11-0096 |
| Wikidata | Q54993518 |
General iPSC Information |
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| Publications | |
| * Is the cell line readily obtainable for third parties? |
No |
| Subclones | |
Donor Information
General Donor Information |
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| Sex | male |
Phenotype and Disease related information (Donor) |
|
| Diseases | |
Ethics
| Alternatives to consent | |
| Alternative consent approval number | NIHhESC-11-0096 |
hESC Derivation
| Embryo stage | Blastula with ICM and Trophoblast |
| ZP removal technique | Enzymatic |
| Cell isolation | Immunosurgery |
| Cell seeding | Isolated ICM |
iPSC Culture Conditions
| Surface coating | Matrigel/Geltrex | ||||||||||||||||||
| Passage method | Mechanically | ||||||||||||||||||
| O2 Concentration | 5 % | ||||||||||||||||||
| CO2 Concentration | 10 % | ||||||||||||||||||
| Medium |
mTeSR™ 1
Supplements
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iPSC Characterisation
Analysis of undifferentiated iPSCs
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| NANOG |
Yes |
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| POU5F1 (OCT-4) |
Yes |
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| ZFP42 (REX-1) |
Yes |
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| TERT |
Yes |
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| SSEA-4 |
Yes |
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| TRA 1-60 |
Yes |
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| TRA 1-81 |
Yes |
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Differentiation Potency
Genotyping
Karyotyping (iPSC Line) |
|
| Has the iPSC line karyotype been analysed? |
Yes
46XY
|
Other Genotyping (iPSC Line) |
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