HPSI0414i-oikd_2

General#

Cell Line

hPSCreg Name WTSIi260-A
Alternative name(s)
HPSI0414i-oikd_2
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 9th May 2018
User feedback
No feedback available yet.

Login to share your feedback, experiences or results with the research community.

Provider

Generator Wellcome Trust Sanger Institute (WTSI)
Distributors

External Databases

HipSci HPSI0414i-oikd_2
BioSamples SAMEA4446772
ECACC 77650380
Cellosaurus CVCL_EE88

General Information

Publications
Projects
Is the cell line available in principle? Available with restrictions: Available for non-commercial research

Donor Information#

General Donor Information

Sex female
Ethnicity White - White British

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA2474460

Ethics#

We are currently revising this section.

hIPSC Derivation#

General

Source cell type
fibroblast
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.
Source cell origin
zone of skin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
  • portion of skin
  • skin zone
  • region of skin
  • skin region
  • skin
show more synonyms
Age of donor (at collection) 40-44
Source cell line vendor Cambridge BioResource

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Notes on reprogramming vector detection CytoTune 2

Vector free reprogramming

Other

Derived under xeno-free conditions
Unkown
Derived under GMP?
Unkown
Available as clinical grade?
Unkown

Culture Conditions#

Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
CO2 Concentration 5 %
Medium TeSR™ E8™

Characterisation#

Analysis of Undifferentiated Cells
Pluripotency Score Novelty Score
29.716 1.283

Certificate of Analysis

Is there a certificate of analysis available?
Yes

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
cnv
http://www.hipsci.org/lines/#/lines/HPSI0414i-oikd_2
Number of regions different from primary tissue: 1; Length of differences from primary tissue: 1
Genotyping array
http://www.ebi.ac.uk/ena/data/view/ERZ368908
Genotyping array calls
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1723699
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ376751
mpileup variant calls
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1861396
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1723715
Raw sequencing reads
Methylation profiling
http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-4059/
Text file with probe intensities
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ376399
BWA alignment
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR1724742
Raw sequencing reads
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR1743829
Raw sequencing reads
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERZ376071
Abundances of transcripts
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ447967
GATK haplotype calls
Genotyping array
http://www.ebi.ac.uk/ena/data/view/ERZ369200
Genotyping array calls
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1702829
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ376575
Imputed and phased genotypes
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERZ376212
Splice-aware STAR alignment