PBMCs were isolated from the donor¡¦s peripheral blood by Ficoll-Paque density-gradient centrifugation. Prior to reprogramming, PBMCs were expanded for 4 days in PBMC expansion medium [StemPro-34 SFM (Thermo Fisher Scientific) supplemented with 2 mM L-glutamine, SCF (100 ng/mL), FLT3 ligand (100 ng/mL), IL-3 (20 ng/mL) and IL-6 (20 ng/mL)]. A total of 5 ¡Ñ 10? PBMCs were reprogrammed using the CytoTuneþ-iPS 2.0 Sendai Reprogramming Kit (Thermo Fisher Scientific) according to the manufacturer¡¦s instructions. A single, well-isolated TRA-1-60¡Vpositive colony was manually picked on day 21 post-transduction and clonally expanded to establish the NTUHi003-A line; the line is therefore of clonal, single-colony origin. Selected colonies were maintained on a mitomycin C¡Vinactivated mouse embryonic fibroblast (MEF) feeder layer seeded on 0.1% gelatin-coated plates in DMEM/F-12 medium supplemented with 20% KnockOutþ Serum Replacement (Gibco), 10 ng/mL basic fibroblast growth factor (bFGF; Gibco), 0.1 mM non-essential amino acids (NEAA; Gibco), 1 mM GlutaMAXþ (Gibco), and 0.1 mM £]-mercaptoethanol (Invitrogen). hiPSCs were cultured at 37¢XC in a humidified incubator with 5% CO?, with daily medium changes, and passaged weekly by manual microdissection at an approximate 1:3 split ratio.