FAQ

What is hPSCreg?

hPSCreg (human Pluripotent Stem Cell registry) is a freely accessible global registry for human pluripotent stem cell lines (hPSC-lines). The registry allows searching for cell lines and for information available about these cell lines. New cell lines can be registered and information to already registered cell lines can be added.

hPSCreg is an open platform for coordination and cooperation in the area of hPSC research and application, to avoid redundancy and promoting comparable quality standards in hPSC research. hPSCreg aims to collaborate with registries and cell banks worldwide.

hPSCreg was established in 2007 and is supported by the European Commission.

Why register cell lines?

Registered cell lines will be widely visible and become part of the global registry of hPSC- lines.

Each line gets a unique identifier based on an international standard nomenclature. The standard name / unique identifier authenticates the lines throughout diverse resources.

Each line gets a BioSamples ID, which connects it to other databases using BioSamples ID’s for biological data (e.g. EBI, NCBI).

Registration provides transparency about the applied ethical procurement and scientific quality of the line using comparable data.

Registered lines can get hPSCreg certificates based on fulfilling minimal criteria of ethical and quality standards.

What is registered?

hPSCreg provides comprehensive information on existing hPSC lines, their ethical procurement, donor characteristics, derivation process, cultivation, pluripotency characteristics, genetic and expression data and other molecular or biological properties, use and quality of the line. The information on each line can be updated, edited and completed. The hPSCreg database is a dynamic database, in that the content is updated through regular surveys conducted by the Steering Committee and feedback from hPSC-line providers. All information collated undergoes a standardized data quality check.

What is a standard hPSCreg name?

A hPSCreg name is assigned to each registered cell line. It is a unique identifier to unambiguously identify a specific hPSC-line throughout diverse resources, publications and databases. More information can be found here and in our publication A Standard Nomenclature for Referencing and Authentication of Pluripotent Stem Cells.

What is a BioSamples ID?

A BioSamples ID is an identifier, which is widely used for biological datasets. It allows to link these datasets when they belong to the same origin, for example a donor of an iPSC-line. BioSamples IDs are used by EBI, NCBI and RIKEN.

What is a hPSCreg certificate?

The hPSCreg certificate for a hPSC-line is provided, when evidence is provided to hPSCreg showing that a set of minimal ethical and scientific standards are met by the line. The ethical standards include documentation of informed consent procedures for the donation of the source material used for derivation of the hPSC-line. Scientific standards include the documented testing of a line’s pluripotency using established and accepted assays. The hPSCreg cell line certificate is essential for some funding agencies. For example, the hPSCreg certificate is essential for the use of a hPSC-line in research funded by the European Union.

Project certificates are provided for research projects registered at hPSCreg.

How can I obtain a cell line certificate?

Already issued certificates from cell lines can be downloaded on the cell line page (for example WAe009-A). If you want to acquire a new certificate, please contact us. Please note, that in order to acquire a cell line certificate, all mandatory information must be given and the cell line must have been validated. Furthermore, we require evidence of pluripotency and a copy of the donor consent form. Please feel free to contact us, if you have further questions.

How can I obtain a project certificate?

If your project has already been provided with a certificate, you can download it on the edit page of the project. If you want to acquire a new certificate, please contact us. Please note, that in order to acquire a project certificate, all project registration fields must be given.

What/who is a generator?

The generator is the institution that generated the cell line. It is not an individual person. Example: A cell line was generated at PSC-University by Dr. Yaka. Consequently the cell line’s generator is the PSC-University and the contact person is Dr. Yaka. The generator of a subclone cell line may differ from the institution that generated this cell line. Each generator must chose an acronym. The generator acronym becomes integral part of the standard name / identifier of all lines registered from that generator. For subclone the acronym of the parental cell line is chosen for the name.

What/who is a distributor?

The distributor is the institution that distributes or sells the cell lines.The distributor may be the same as the generator or owner, but it can also be a different institution such as a cell line bank or a company.

What/who is an owner?

The owner is the person/institution who owns the property rights for the cell line.

What is a subclone?

A subclone in hPSCreg is a derivative of a specific parental cell line. A subclone is distinct from the parental line because it carries a stable defined genetic modification, which was induced (e.g. transgene) or occurred spontaneously (e.g. mutation) in the parental line. Naturally, parental line and subclone have the same human donor.

How do I register a new cell line?

  1. After logging in, you can go to your dashboard and select Register cell line.
  2. Choose the generator from the list or create a new generator (see How do I register a new institution?).
  3. Choose thy type of the cell line (hESC or hiPSC).
  4. If the cell line is a subclone of another cell line or other cell lines from the same source material have already registered, select the respective cell line from the drop down list.
  5. You can add alternative names like the name used for your cell line internally.
  6. You can add additional comments for other editors and the validator.
  7. After clicking on finish, the line will be registered and a standardized cell line name will be created. Further details can and should be added when editing the cell line.

How do I get a login for hPSCreg to register or modify a line?

Go to the hPSCreg homepage (https://hpscreg.eu/). Click the login icon at the top right and click ‘sign up’. Follow the instructions.

I forgot my password. What can I do?

Enter the e-mail address you used to register at hESCreg.eu here. You will get an email which allows you to set a new password.

How can I get access to register lines for an already registered institution?

  1. After logging in, you can go to your dashboard and select My institutions.
  2. There you have two possibilities: Find and assign institution and Register and assign a new institution.
  3. Click on Find and assign institution.
  4. A list appears where you can search for your institution.
  5. You can leave a comment for the institution managers to explain, why do you need access to the institution.
  6. After clicking on Assign institution, the hPSCreg manager for this institution will be informed about your request and can accept or decline it.
  7. If you don’t get an answer within three days, please contact us.

Who is the hPSCreg manager of an institution?

Usually, the person registering a new institution becomes per default the hPSCreg manager of that institution. The hPSCreg manager can accept or decline access requests from other users. The institutional hPSCreg manager can also appoint additional institutional hPSCreg managers, or withdraw as hPSCreg manager. However, each registered institution must have at least one hPSCreg manager.

How do I register a new institution?

  1. If you can’t find your institution in the list of already registered institutions (See How can I get access to register lines for an already registered institution?) you can register a new institution.
  2. After logging in, you can go to your dashboard and select My institutions.
  3. There you have two possibilities: Find and assign institution and Register and assign a new institution.
  4. Click on Register and assign a new institution.
  5. Enter the name and address data.
  6. You can also optionally select a higher-level institution or company , e.g. "University of Springfield", if your institution is a sub-institution like "Institute for Stem Cell Research" or a laboratory like ‘Embryology Lab’. The higher-level institution need to be registered first.
  7. You have to select a generator acronym for the institution if you want to register cell lines for this institution. Institutions without acronym can not be used as generator.
  8. After submission, you are the manager of the institution and can register cell lines for this institution.

What is a generator acronym?

A generator acronym is a unique institutional code of 2-6 (uppercase) letters. Each (sub)institution registering cell lines can self-assign an acronym. Only institutions having such an acronym can be set as generator for a new cell line. The acronym must be unique in hPSCreg. Even subinstutitions are not allowed to have the same acronym as their parent institution.

The generator acronym is an integral part of the hPSCreg standard name / unique identifier. More information can be found here and in our publication A Standard Nomenclature for Referencing and Authentication of Pluripotent Stem Cells You can set the parental institution as the generator to use the corresponding acronym and set your subinstitution as the contact institution.

How do I register a publication?

  1. After logging in, you can go to your dashboard and select Register publication.
  2. You can add the publication using a pubmed, PMC or DOI.
  3. Or you can enter the publication data manually.
  4. Registered cell lines and research projects can be assigned to the publication.

How do I register a research project?

  1. After logging in, you can go to your dashboard and select Register project.
  2. Add title, acronym, summary, a link to the webpage of the project, start and end date.
  3. You can select a sponsor for the project from the list or enter a new one.
  4. Add an institution to the project (see also How do I register a new institution?).
  5. Add contact details of the project investigator or use your own account data.
  6. Decide whether contact details should be public.
  7. Assign cell lines you are going to use in your project.
  8. Save the data

When will my cell lines become public?

Directly after the registration the name of the cell line, the generator institution, alternative name(s), the cell line type, date of the last update and potential user feedback will be visible. All other data will not be shown on the public page until the cell line has been submitted by the user. Only when all mandatory fields have been given, the line can be validated by hPSCreg. Validation is a check for completeness of data provision. For validated cell lines all information, except information that has been marked as private, is shown on the public cell line page.

How can I provide feedback for a cell line?

Registered hPSCreg users can give public feedback, like experiences or results within the research community, for each cell line. When logged in, you can provide comments directly within the cell line details page in the General section.

What are mandatory information for cell line registration?

Minimal information for cell line registration includes:

  • Documentation of ethical provenance of the cells according to national regulation
  • Evidence for pluripotency of the cell
  • Principal availability of the cell line for research
A detailed list of mandatory information can be found in this document.