Publications associated with registered cell lines

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  1. Adamo A

    7q11.23 dosage-dependent dysregulation in human pluripotent stem cells affects transcriptional programs in disease-relevant lineages.

    10th Feb 2016

    Journal etc.

    PubMed: 25501393 DOI: 10.1038/ng.3169
    Associated cell lines:
  2. Lim ML

    Decellularized feeders: an optimized method for culturing pluripotent cells.

    10th Feb 2016

    Journal etc.

    PubMed: 24167316 DOI: 10.5966/sctm.2013-0077
    Associated cell lines:
  3. Jenny

    Preimplantation Genetic Screening

    15th Apr 2015

    Journal etc.

    PubMed: DOI:
    Associated cell lines:
  4. Shao K

    Induced pluripotent mesenchymal stromal cell clones retain donor-derived differences in DNA methylation profiles.

    11th Mar 2015

    Journal etc.

    PubMed: 23032973 DOI: 10.1038/mt.2012.207
    Associated cell lines:
  5. Koch CM

    Pluripotent stem cells escape from senescence-associated DNA methylation changes.

    11th Mar 2015

    Journal etc.

    PubMed: 23080539 DOI: 10.1101/gr.141945.112
    Associated cell lines:
  6. Frobel J

    Epigenetic rejuvenation of mesenchymal stromal cells derived from induced pluripotent stem cells.

    11th Mar 2015

    Journal etc.

    PubMed: 25241740 DOI: 10.1016/j.stemcr.2014.07.003
    Associated cell lines:
  7. Zhang XH

    Ca2+ signaling in human induced pluripotent stem cell-derived cardiomyocytes (iPS-CM) from normal and catecholaminergic polymorphic ventricular tachycardia (CPVT)-afflicted subjects.

    11th Mar 2015

    Journal etc.

    PubMed: 23684427 DOI: 10.1016/j.ceca.2013.04.004
    Associated cell lines:
  8. Fatima A

    In vitro modeling of ryanodine receptor 2 dysfunction using human induced pluripotent stem cells.

    11th Mar 2015

    Journal etc.

    PubMed: 22178870 DOI: 10.1159/000335753
    Associated cell lines:
  9. Fatima A

    The disease-specific phenotype in cardiomyocytes derived from induced pluripotent stem cells of two long QT syndrome type 3 patients.

    11th Mar 2015

    Journal etc.

    PubMed: 24349418 DOI: 10.1371/journal.pone.0083005
  10. Desmarais JA

    Apoptosis and failure of checkpoint kinase 1 activation in human induced pluripotent stem cells under replication stress.

    22nd Jan 2015

    Journal etc.

    PubMed: 26810087 DOI: 10.1186/s13287-016-0279-2
    Associated cell lines:
  11. Dr. Anna Veiga

    Reprogramming of human fibroblasts to induce pluripotent stem cells under xeno-free conditions

    9th Sep 2010

    Journal etc.

    PubMed: 19890879 DOI: 10.1002/stem.248

    Abstract:

    The availability of induced pluripotent stem cells (iPSCs) has created extraordinary opportunities for modeling and perhaps treating human disease. However, all reprogramming protocols used to date involve the use of products of animal origin. Here, … Show all

    Associated cell lines:
  12. MD John DE VOS

    A gene expression signature shared by human mature oocytes and embryonic stem cells

    28th May 2010

    Journal etc.

    PubMed: 19128516 DOI: 10.1186/1471-2164-10-10

    Abstract:

    BACKGROUND: The first week of human pre-embryo development is characterized by the induction of totipotency and then pluripotency. The understanding of this delicate process will have far reaching implication for in vitro fertilization and regenerati… Show all

    Associated cell lines:
  13. Steven Shoei-Lung Li

    Identification of microRNAs regulated by activin A in human embryonic stem cells.

    10th Mar 2010

    Journal etc.

    PubMed: 19885849 DOI: 10.1002/jcb.22385

    Abstract:

    Human embryonic stem (hES) cells have the capacities to propagate for extended periods and to differentiate into cell types from all three germ layers both in vitro and in vivo. These characteristics of self-renewal and pluripotency enable hES cells … Show all

    Associated cell lines:
  14. Dr. Anna Veiga

    Desease corrected haematopoietic progenitors from Fanconi anemia induced pluripotent stem cells

    22nd Feb 2010

    Journal etc.

    PubMed: 19483674 DOI: 10.1038/nature08129

    Abstract:

    The generation of induced pluripotent stem (iPS) cells has enabled the derivation of patient-specific pluripotent cells and provided valuable experimental platforms to model human disease. Patient-specific iPS cells are also thought to hold great the… Show all

    Associated cell lines:
  15. Prof. Petr Dvorak

    Lack of Population Diversity in Human Embryonic Stem-Cell Lines

    17th Feb 2010

    Journal etc.

    PubMed: 20018958 DOI: 10.1056/NEJMc0910371

    Abstract:

    We determined the genetic ancestry of a large collection of stem-cell lines. We have found that widely distributed stem-cell lines lack population diversity and that none of these lines derive from populations with recent African ancestry. Efforts t… Show all

  16. Prof. Petr Dvorak

    Consensus guidance for banking and supply of human embryonic stem cell lines for research purposes

    17th Feb 2010

    Journal etc.

    PubMed: 20016957 DOI: 10.1007/s12015-009-9085-x

    Abstract:

    In just a few years hundreds of human embryonic stem cell (hESC) lines have been established in laboratories around the world and many programmes of research initiated to investigate their properties and broad ranging potential in therapy and for oth… Show all

  17. Prof. Dr. Marisa Jaconi

    Derivation of human embryonic stem cell lines from single cells of 4-cell stage embryos: be aware of the risks.

    16th Feb 2010

    Journal etc.

    PubMed: 18854403 DOI: 10.1093/humrep/den369
    Associated cell lines:
  18. Prof. Dr. Outi Hovatta

    Efficient production of mesencephalic dopamine neurons by Lmx1a expression in embryonic stem cells

    15th Feb 2010

    Journal etc.

    PubMed: 19383789 DOI: 10.1073/pnas.0902396106

    Abstract:

    Signaling factors involved in CNS development have been used to control the differentiation of embryonic stem cells (ESCs) into mesencephalic dopamine (mesDA) neurons, but tend to generate a limited yield of desired cell type. Here we show that force… Show all

    Associated cell lines:
  19. Prof. Dr. Outi Hovatta

    Neurotrophic factors promote cholinergic differentiation in human embryonic stem cell derived neurons

    15th Feb 2010

    Journal etc.

    PubMed: 19799651 DOI: 10.1111/j.1582-4934.2009.00916.x

    Abstract:

    Cholinergic neurotransmission is essential for many important functions in the brain, including cognitive mechanisms. Here we demonstrate that human embryonic stem (hES) cells differentiate into a population of neuronal cells that express the choline… Show all

    Associated cell lines:
  20. Prof. Dr. Outi Hovatta

    transplantation of human embryonic stem cell-derived neural precursor cells and enriched environment after cortical stroke in rats: cell survival and functional recovery

    15th Feb 2010

    Journal etc.

    PubMed: 19175403 DOI: 10.1111/j.1460-9568.2008.06599.x

    Abstract:

    Cortical stem cell transplantation may help replace lost brain cells after stroke and improve the functional outcome. In this study, we transplanted human embryonic stem cell (hESC)-derived neural precursor cells (hNPCs) or vehicle into the cortex of… Show all

    Associated cell lines:
  21. Prof. Dr. Outi Hovatta

    Liver X receptors and oxysterols promote ventral midbrain neurogenesis in vivo and in human embryonic stem cells

    15th Feb 2010

    Journal etc.

    PubMed: 19796621 DOI: 10.1016/j.stem.2009.08.019

    Abstract:

    Control over progenitor proliferation and neurogenesis remains a key challenge for stem cell neurobiology and a prerequisite for successful stem cell replacement therapies for neurodegenerative diseases like Parkinson's disease (PD). Here, we examine… Show all

    Associated cell lines:
  22. Prof. Dr. Outi Hovatta

    CD marker expression profiles of human embryonic stem cells and their neural derivatives, determined using flow-cytometric analysis, reveal a novel CD marker for exclusion of pluripotent stem cells

    15th Feb 2010

    Journal etc.

    PubMed: 19383417 DOI: 10.1016/j.scr.2008.08.001

    Abstract:

    Human embryonic stem cells (hESCs) are pluripotent cells that can differentiate into neural cell lineages. These neural populations are usually heterogeneous and can contain undifferentiated pluripotent cells that are capable of producing teratomas i… Show all

  23. Prof. Norio Nakatsuji

    Characterization of human embryonic stem cell lines by the International Stem Cell Initiative.

    15th Feb 2010

    Journal etc.

    PubMed: 17572666 DOI: 10.1038/nbt1318

    Abstract:

    The International Stem Cell Initiative characterized 59 human embryonic stem cell lines from 17 laboratories worldwide. Despite diverse genotypes and different techniques used for derivation and maintenance, all lines exhibited similar expression pat… Show all

  24. Prof. Dr. Outi Hovatta

    A gene expression signature shared by human mature oocytes and embryonic stem cells

    15th Feb 2010

    Journal etc.

    PubMed: 19128516 DOI: 10.1186/1471-2164-10-10

    Abstract:

    BACKGROUND: The first week of human pre-embryo development is characterized by the induction of totipotency and then pluripotency. The understanding of this delicate process will have far reaching implication for in vitro fertilization and regenerati… Show all

    Associated cell lines:
  25. Prof. Dr. Outi Hovatta

    Costimulation blockade induces tolerance to HESC transplanted to the testis and induces regulatory T-cells to HESC transplanted into the heart.

    15th Feb 2010

    Journal etc.

    PubMed: 18467659 DOI: 10.1634/stemcells.2008.0111

    Abstract:

    In order to study the ability of costimulation blockade to induce tolerance to human embryonic stem cells (HESC), severe combined immunodeficient (SCID), and immunocompetent C57BL/6 mice treated with costimulation blockade received intratesticular an… Show all

    Associated cell lines:
  26. Prof. Dr. Outi Hovatta

    Cryopreservation of dissociated human embryonic stem cells in the presence of ROCK inhibitor

    15th Feb 2010

    Journal etc.

    PubMed: 19585460 DOI: 10.1002/9780470151808.sc01c08s10

    Abstract:

    Two different methods have been adopted for the cryopreservation of human embryonic stem cells (hESCs): vitrification and conventional slow freezing/rapid thawing. However, these methods present poor viability and high differentiation rates. Therefor… Show all

    Associated cell lines:
  27. Dr Susan Kimber

    Derivation of Man-1 and Man-2 research grade human embryonic stem cell lines

    14th Feb 2010

    Journal etc.

    PubMed: 20177994 DOI: 10.1007/s11626-010-9291-5

    Abstract:

    We report here the derivation of two new human embryonic stem cell lines, Man-1 and Man-2, and their full characterization as novel pluripotent stem cell lines. Man-1 was derived from an embryo surplus to requirement from routine IVF, while Man-2 was… Show all

    Associated cell lines:
  28. Prof. Norio Nakatsuji

    Efficient establishment of human embryonic stem cell lines and long-term maintenance with stable karyotype by enzymatic bulk passage.

    11th Feb 2010

    Journal etc.

    PubMed: 16707099 DOI: 10.1016/j.bbrc.2006.04.135

    Abstract:

    Human ES (hES) cell lines are considered to be a valuable resource for medical research and for applications in cell therapy and drug discovery. For such utilization of hES cells to be realized, however, protocols involved in the use of hES cells, su… Show all

  29. Prof. Petr Dvorak

    A complex role for FGF-2 in self renewal, survival, and adhesion of human embryonic stem cells

    10th Feb 2010

    Journal etc.

    PubMed: 19544431 DOI: 10.1002/stem.128

    Abstract:

    The transcription program that is responsible for the pluripotency of human ESCs (hESCs) is believed to be comaintained by exogenous fibroblast growth factor-2 (FGF-2), which activates FGF receptors (FGFRs) and stimulates the mitogen-activated protei… Show all

    Associated cell lines:
  30. Prof. Petr Dvorak

    Proteomic profiling of human embryonic stem cell-derived microvesicles reveals a risk of transfer of proteins of bovine and mouse origin

    10th Feb 2010

    Journal etc.

    PubMed: 19401887 DOI: 10.1080/14653240802595531

    Abstract:

    Microvesicles (MVs) shed from the plasma membrane of eukaryotic cells including human embryonic stem cells (hESCs) contain proteins, lipids, and RNAs and serve as mediators of cell-to-cell communication. However, they may also contain immunogenic mem… Show all

    Associated cell lines:
  31. Xiaofang Sun

    Similar biological characteristics of human embryonic stem cell lines with normal and abnormal karyotypes.

    9th Feb 2010

    Journal etc.

    PubMed: 18611919 DOI: 10.1093/humrep/den137

    Abstract:

    BACKGROUND: Human embryonic stem cell (hESC) lines derived from poor quality embryos usually have either normal or abnormal karyotypes. However, it is still unclear whether their biological characteristics are similar. METHODS: Seven new hESC lines w… Show all

    Associated cell lines:
  32. Prof. Dr. Outi Hovatta

    Immortalized human skin fibroblast feeder cells support growth and maintenance of both human embryonic and induced pluripotent stem cells

    29th Jun 2009

    Journal etc.

    PubMed: 19556288 DOI: 10.1093/humrep/dep232

    Abstract:

    BACKGROUND Feeder cells are frequently used for the early-stage of derivation and culture of human embryonic stem cell (hESC) lines. METHODS We established a conditionally immortalized human foreskin fibroblast line that secreted basic fibroblast gro… Show all

    Associated cell lines:
  33. Hui Z. Sheng

    Human embryonic stem cell lines derived from the Chinese population

    24th Feb 2009

    Journal etc.

    PubMed: 15916726 DOI: 10.1038/sj.cr.7290307

    Abstract:

    Six human embryonic stem cell lines were established from surplus blastocysts. The cell lines expressed alkaline phosphatase and molecules typical of primate embryonic stem cells, including Oct-4, Nanog, TDGF1, Sox2, EBAF, Thy-1, FGF4, Rex-1, SSEA-3,… Show all

  34. Dr. Claus Yding Andersen

    Human Embryonic Stem Cells in Culture Possess Primary Cilia

    23rd Feb 2009

    Journal etc.

    PubMed: 18332216 DOI: 10.1083/jcb.200706028

    Abstract:

    Human embryonic stem cells (hESCs) are potential therapeutic tools and models of human development. With a growing interest in primary cilia in signal transduction pathways that are crucial for embryological development and tissue differentiation and… Show all

    Associated cell lines:
  35. Dr. Anna Veiga

    Efficient and rapid generation of induced pluripotent stem cells from human keratinocytes

    22nd Dec 2008

    Journal etc.

    PubMed: 18931654 DOI: 10.1038/nbt.1503

    Abstract:

    The utility of induced pluripotent stem (iPS) cells for investigating the molecular logic of pluripotency and for eventual clinical application is limited by the low efficiency of current methods for reprogramming. Here we show that reprogramming of … Show all

    Associated cell lines:
  36. Dr. Anna Veiga

    Generation of cardiomyocytes from new human embryonic stem cell lines derived from poor quality blastocysts

    22nd Dec 2008

    Journal etc.

    PubMed: 19028986 DOI: 10.1101/sbq.2008.73.038

    Abstract:

    Human embryonic stem (hES) cells represent a potential source for cell replacement therapy of many degenerative diseases. Most frequently, hES cell lines are derived from surplus embryos from assisted reproduction cycles, independent of their quality… Show all

  37. Prof. Dr. Lorraine Young

    Gene-specific vulnerability to imprinting variability in human embryonic stem cell lines

    4th Dec 2008

    Journal etc.

    PubMed: 17989250 DOI: 10.1101/gr.6609207

    Abstract:

    Disregulation of imprinted genes can be associated with tumorigenesis and altered cell differentiation capacity and so could provide adverse outcomes for stem cell applications. Although the maintenance of mouse and primate embryonic stem cells in a … Show all

    Associated cell lines:
  38. Prof. Dr. Lorraine Young

    Improved human embryonic stem cell embryoid body homogeneity and cardiomyocyte differentiation from a novel V-96 plate aggregation system highlights interline variability.

    4th Dec 2008

    Journal etc.

    PubMed: 17185609 DOI: 10.1634/stemcells.2006-0598

    Abstract:

    Although all human ESC (hESC) lines have similar morphology, express key pluripotency markers, and can differentiate toward primitive germ layers in vitro, the lineage-specific developmental potential may vary between individual lines. In the current… Show all

    Associated cell lines:
  39. Prof. Dr. Lorraine Young

    Restriction landmark genome scanning identifies culture-induced DNA methylation instability in the human embryonic stem cell epigenome.

    4th Dec 2008

    Journal etc.

    PubMed: 17409196 DOI: 10.1093/hmg/ddm074

    Abstract:

    Widespread provision of human embryonic stem cells (hESCs) for therapeutic use, drug screening and disease modelling will require cell lines sustainable over long periods in culture. Since the short-term, in vitro culture of mammalian embryos can res… Show all

    Associated cell lines:
  40. Prof. Dr. Lorraine Young

    Improved genetic manipulation of human embryonic stem cells.

    4th Dec 2008

    Journal etc.

    PubMed: 18391958 DOI: 10.1038/nmeth.1200

    Abstract:

    Low efficiency of transfection limits the ability to genetically manipulate human embryonic stem cells (hESCs), and differences in cell derivation and culture methods require optimization of transfection protocols. We transiently transferred multiple… Show all

    Associated cell lines:
  41. Prof. Dr. Lorraine Young

    Feeder-free culture of human embryonic stem cells in conditioned medium for efficient genetic modification.

    4th Dec 2008

    Journal etc.

    PubMed: 18772870 DOI: 10.1038/nprot.2008.140

    Abstract:

    Realizing the potential of human embryonic stem cells (hESCs) in research and commercial applications requires generic protocols for culture, expansion and genetic modification that function between multiple lines. Here we describe a feeder-free hESC… Show all

    Associated cell lines:
  42. Prof. Dr. Lorraine Young

    Feeder-free culture of human embryonic stem cells in conditioned medium for efficient genetic modification.

    4th Dec 2008

    Journal etc.

    PubMed: 18772870 DOI: 10.1038/nprot.2008.140

    Abstract:

    Realizing the potential of human embryonic stem cells (hESCs) in research and commercial applications requires generic protocols for culture, expansion and genetic modification that function between multiple lines. Here we describe a feeder-free hESC… Show all

    Associated cell lines:
  43. Andy Peng Xiang

    Establishment and characterization of two new human embryonic stem cell lines, SYSU-1 and SYSU-2.

    4th Nov 2008

    Journal etc.

    PubMed: 17442208

    Abstract:

    Human embryonic stem cells can propagate indefinitely in vitro and are able to differentiate into derivatives of all three embryonic germ layers. The excitement surrounding human embryonic stem cells lies largely in their potential to produce special… Show all

    Associated cell lines:
  44. Dr. Carlos Simón Valles

    Derivation and characterization of three new Spanish human embryonic stem cell lines (VAL -3 -4 -5) on human feeder and in serum-free conditions.

    4th Nov 2008

    Journal etc.

    PubMed: 17169214

    Abstract:

    A total of 184 human embryos, frozen for >5 years, were donated; informed consent was obtained according to Spanish law 45/2003. Survival rate was 40% and three out of 24 blastocysts (12.5%) developed into putative hESC lines, named VAL-3, VAL-4, … Show all

    Associated cell lines:
  45. Prof. Dr. Bernie Tuch

    Derivation of three clones from human embryonic stem cell lines by FACS sorting and their characterization.

    4th Nov 2008

    Journal etc.

    PubMed: 16522163 DOI: 10.1089/scd.2006.15.61

    Abstract:

    Here we describe the first report of three human embryonic stem cell (hESC) clones, hES 3.1, 3.2, and 3.3, derived from the parent line hES3 by sorting of single-cell preparations by flow cytometry. The viability of single-cell preparations before an… Show all

  46. Renee Pera

    Characterization of six new human embryonic stem cell lines (HSF7, -8, -9, -10, -12, and -13) derived under minimal-animal component conditions.

    31st Oct 2008

    Journal etc.

    PubMed: 18513167 DOI: 10.1089/scd.2007.0216

    Abstract:

    Human embryonic stem cells (hESCs) provide a renewable source of a variety of cell types with the potential for use in both scientific research and clinical cell-based therapy. Several hESC lines have previously been isolated and characterized, howev… Show all

  47. Prof. Dr. James A. Thompson

    Derivation of human embryonic stem cells in defined conditions.

    31st Oct 2008

    Journal etc.

    PubMed: 16388305 DOI: 10.1038/nbt1177

    Abstract:

    We have previously reported that high concentrations of basic fibroblast growth factor (bFGF) support feeder-independent growth of human embryonic stem (ES) cells, but those conditions included poorly defined serum and matrix components. Here we repo… Show all

    Associated cell lines:
  48. Shinya Yamanaka

    Induction of pluripotent stem cells from adult human fibroblasts by defined factors.

    13th Oct 2008

    Journal etc.

    PubMed: 18035408 DOI: 10.1016/j.cell.2007.11.019

    Abstract:

    Successful reprogramming of differentiated human somatic cells into a pluripotent state would allow creation of patient- and disease-specific stem cells. We previously reported generation of induced pluripotent stem (iPS) cells, capable of germline t… Show all

  49. Dr. Allan Robins

    Humanic Embryonic Stem Cell Lines Derived from Discarded Embryos

    13th Oct 2008

    Journal etc.

    PubMed: 12968106 DOI: 10.1634/stemcells.21-5-521

    Abstract:

    Human pluripotent embryonic stem (ES) cells have important potential in regenerative medicine and as models for human preimplantation development; however, debate continues over whether embryos should be destroyed to produce human ES cells. We have d… Show all

  50. Dr. Hossein Baharvand

    Establishment and in vitro differentiation of a new embryonic stem cell line from human blastocyst

    13th Oct 2008

    Journal etc.

    PubMed: 15270778 DOI: 10.1111/j.1432-0436.2004.07205005.x

    Abstract:

    Embryonic stem cells have the ability to remain undifferentiated and proliferate indefinitely in vitro while maintaining the potential to differentiate into derivatives of all three embryonic germ layers. These cells have, therefore, potential for in… Show all

    Associated cell lines:
  51. Prof. Dr. Bernie Tuch

    Derivation of a new human embryonic stem cell line, endeavour-1, and its clonal propagation

    13th Oct 2008

    Journal etc.

    PubMed: 18271699 DOI: 10.1089/scd.2007.0055

    Abstract:

    Here we describe the derivation of a novel human embryonic stem (hES) cell line, Endeavour-1 (E1), its four new clonal lines (E1C1, E1C2, E1C3, E1C4), and their characterization. E1 and its clonal lines are propagated on human fetal fibroblasts (HFFs… Show all

    Associated cell lines:
  52. Dr. Derek Hei

    Embryonic stem cell lines derived from human blastocysts

    13th Oct 2008

    Journal etc.

    PubMed: 9804556

    Abstract:

    Human blastocyst-derived, pluripotent cell lines are described that have normal karyotypes, express high levels of telomerase activity, and express cell surface markers that characterize primate embryonic stem cells but do not characterize other earl… Show all

  53. Dr. Hossein Baharvand

    Generation of new human embryonic stem cell lines with diploid and triploid karyotypes.

    7th Oct 2008

    Journal etc.

    PubMed: 16512855 DOI: 10.1111/j.1440-169x.2006.00851.x

    Abstract:

    Human pluripotent embryonic stem cells (hESC) have great promise for research into human developmental biology and the development of cell therapies for the treatment of diseases. To meet the increased demand for characterized hESC lines, we presen… Show all

  54. Prof. Dr. Vladimir Zachar

    Derivation and characterization of four new human embryonic stem cell lines: the Danish experience.

    6th Oct 2008

    Journal etc.

    PubMed: 16454946

    Abstract:

    In September 2003, legislation approved in Denmark legalized work on surplus human embryos from IVF for clinical purposes to establish human embryonic stem (ES) cell cultures. The aim of this study was to establish such stem cell lines. Fresh surplus… Show all

  55. Prof. Christine Mummery

    Insulin Redirects Differentiation from Cardiogenic Mesoderm and Endoderm to Neuroectoderm in Differentiating Human Embryonic Stem Cells

    29th Sep 2008

    Journal etc.

    PubMed: 18096723 DOI: 10.1634/stemcells.2007-0617

    Abstract:

    Human embryonic stem cells (hESC) can proliferate indefinitely while retaining the capacity to form derivatives of all three germ layers. We have reported previously that hESC differentiate into cardiomyocytes when cocultured with a visceral endoderm… Show all

    Associated cell lines:
  56. Prof. Christine Mummery

    Human embryonic stem cells: towards therapies for cardiac disease. Derivation of a Dutch human embryonic stem cell line

    29th Sep 2008

    Journal etc.

    PubMed: 16274613 DOI:

    Abstract:

    Cell transplantation is being discussed as a potential therapy for multiple disorders caused by loss or malfunction of single or at most a few cell types. These include diabetes, Parkinson·s disease and myocardial infarction or cardiac failur… Show all

    Associated cell lines:
  57. Prof. Christine Mummery

    Improved genetic manipulation of human embryonic stem cells

    29th Sep 2008

    Journal etc.

    PubMed: 18391958 DOI: 10.1038/NMETH.1200

    Abstract:

    Low efficiency of transfection limits the ability to genetically manipulate human embryonic stem cells (hESCs), and differences in cell derivation and culture methods require optimization of transfection protocols. We transiently transferred multiple… Show all

    Associated cell lines:
  58. Prof. Dr, Karen Sermon

    Efficient differentiation of human embryonic stem cells into a homogeneous population of osteoprogenitor-like cells.

    22nd Sep 2008

    Journal etc.

    PubMed: 18492382

    Abstract:

    The use of human embryonic stem cells (hESC) in both research and therapeutic applications requires relatively large homogeneous populations of differentiated cells. The differentiation of three hESC lines into highly homogeneous populations of osteo… Show all

    Associated cell lines:
  59. Prof. Dr, Karen Sermon

    Epithelial-mesenchymal transition process in human embryonic stem cells cultured in feeder-free conditions.

    22nd Sep 2008

    Journal etc.

    PubMed: 17090644 DOI: 10.1093/molehr/gal091

    Abstract:

    Feeder-free human embryonic stem cell (hESC) culture is associated with the presence of mesenchymal-like cells appearing at the periphery of the colonies. The aim of this study was to identify this early differentiation process. Long-term feeder-free… Show all

    Associated cell lines:
  60. Prof. Dr, Karen Sermon

    GSK-3-specific inhibitor-supplemented hESC medium prevents the epithelial-mesenchymal transition process and the up-regulation of matrix metalloproteinases in hESCs cultured in feeder-free conditions.

    22nd Sep 2008

    Journal etc.

    PubMed: 18263607 DOI: 10.1093/molehr/gan001

    Abstract:

    Feeder-free culture induces spontaneous differentiation of human embryonic stem cells (hESCs), identified as an epithelial to mesenchymal transition (EMT). The maintenance of pluripotency of hESCs in feeder-free cultures through the activation of the… Show all

  61. Prof. Dr, Karen Sermon

    CTG repeat instability in a human embryonic stem cell line carrying the myotonic dystrophy type 1 mutation.

    22nd Sep 2008

    Journal etc.

    PubMed: 18577525 DOI: 10.1093/molehr/gan034

    Abstract:

    Human embryonic stem cells (hESC) are considered to be an indefinite source of self-renewing cells that can differentiate into all types of cells of the human body and could be used in regenerative medicine, drug discovery and as a model for studying… Show all

  62. Prof. Dr. Marisa Jaconi

    Derivation of the first Swiss human embryonic stem cell line from a single blastomere of an arrested four-cell stage embryo

    18th Sep 2008

    Journal etc.

    PubMed: 18803035 DOI: 2008/37/smw-12385

    Abstract:

    Summary Principles: Human embryonic stem cells (hESC) hold huge potential for regenerative medicine. So far, the majority of hESC lines have been derived from the isolated inner cell mass (ICM) of blastocysts of variable quality, and several of the… Show all

    Associated cell lines:
  63. Dr. Stephen Minger

    Generation of a human embryonic stem cell line encoding the cystic fibrosis mutation F508, using preimplantaion genetic diagnosis

    19th Aug 2008

    Journal etc.

    PubMed: 15820050

    Abstract:

    Human embryonic stem (hES) cells are pluripotent cells isolated from early human embryos. They can be grown in vitro and made to differentiate into many different cell types. These properties have suggested that they may be useful in cell replacement… Show all

    Associated cell lines:
  64. Dr. Necati Findikli

    Establishment and characterization of new human embryonic stem cell lines

    18th Aug 2008

    Journal etc.

    PubMed: 15949219

    Abstract:

    Human embryonic stem cells (hESC), with their ability to differentiate into all cell types in the human body, are likely to play a very important therapeutic role in a variety of neurodegenerative and life-threatening disorders in the near future. Al… Show all

    Associated cell lines:
  65. Dr. Allan Robins

    A variant human embryonic stem cell line exhibits rapid growth after passaging and reliable dopaminergic differentiation

    8th Aug 2008

    Journal etc.

    PubMed: 15798361

    Abstract:

    Purpose: To explore a karyotypically abnormal variant human embryonic stem cell (hESC) line, BG01V, as a potential model for studies of dopaminergic neuronal differentiation. Methods: The properties of BG01V cells were compared to those of normal … Show all

    Associated cell lines:
  66. Prof. Dr. Benjamin Reubinoff

    Laser-assisted derivation of human embryonic stem cell lines from IVF embryos after preimplantation genetic diagnosis.

    4th Aug 2008

    Journal etc.

    PubMed: 17989069 DOI: 10.1093/humrep/dem351

    Abstract:

    BACKGROUND: Human embryonic stem cells (hESCs) suitable for future transplantation therapy should preferably be developed in an animal-free system. Our objective was to develop a laser-based system for the isolation of the inner cell mass (ICM) that … Show all

  67. M.Sc Zafer Nihat Candan

    Establishment and characterization of new human embryonic stem cell lines

    18th May 2008

    Journal etc.

    PubMed: 15949219

    Abstract:

    Human embryonic stem cells (hESC), with their ability to differentiate into all cell types in the human body, are likely to play a very important therapeutic role in a variety of neurodegenerative and life-threatening disorders in the near future. Al… Show all

    Associated cell lines:
  68. Prof. Dr. Outi Hovatta

    Comparative genomic hybridisation and karyptyping of human embryonic stem cells reveals the occurrence of an isodicentric X chromosome after long-term cultivation

    20th Feb 2008

    Journal etc.

    PubMed: 15044603 DOI: 10.1093/molehr/gah051

    Abstract:

    Human embryonic stem (hES) cells are important research tools in studies of the physiology of early tissue differentiation. In addition, prospects are high regarding the use of these cells for successful cell transplantation. However, one concern has… Show all

    Associated cell lines:
  69. Prof. Dr. Outi Hovatta

    Monitoring and analysis of dynamic growth of human embryonic stem cells: comparison of automated instrumentation and conventional culturing methods.

    20th Feb 2008

    Journal etc.

    PubMed: 17428350 DOI: 10.1186/1475-925X-6-11

    Abstract:

    BACKGROUND: Human embryonic stem cells (hESCs) are a potential source of cells for use in regenerative medicine. Automation of culturing, monitoring and analysis is crucial for fast and reliable optimization of hESC culturing methods. Continuous moni… Show all

    Associated cell lines:
  70. Prof. Dr. Outi Hovatta

    Characterization of human embryonic stem cell lines by the International Stem Cell Initiative.

    20th Feb 2008

    Journal etc.

    PubMed: 17572666 DOI: 10.1038/nbt1318

    Abstract:

    The International Stem Cell Initiative characterized 59 human embryonic stem cell lines from 17 laboratories worldwide. Despite diverse genotypes and different techniques used for derivation and maintenance, all lines exhibited similar expression pat… Show all

  71. Prof. Dr. Outi Hovatta

    In vitro neural differentiation of human embryonic stem cells

    20th Feb 2008

    Journal etc.

    PubMed: 15601586

    Abstract:

    Human embryonic stem (hES) cells are seen as a source of defined neural cell population for pharmaceutical screening or transplantation and can be conducted to differentiate in vitro to mature neurons [1]. hES cells from line HS 237T (Karolinska … Show all

    Associated cell lines:
  72. Prof. Dr. Outi Hovatta

    Mechanical isolation of the inner cell mass is effective in derivation of new human embryonic stem cell lines.

    20th Feb 2008

    Journal etc.

    PubMed: 17959612 DOI: 10.1093/humrep/dem335

    Abstract:

    BACKGROUND: For clinical grade human embryonic stem cell (hESC) lines, a robust derivation system without any substances having animal origin would be required. We have gradually improved our hESC derivations. Human skin fibroblasts were used as feed… Show all

  73. Prof. Dr. Outi Hovatta

    Neurogenic neuroepithelial and radial glial cells generated from six human embryonic stem cell lines in serum-free suspension and adherent cultures.

    20th Feb 2008

    Journal etc.

    PubMed: 17152062 DOI: 10.1002/glia.20463

    Abstract:

    The great potential of human embryonic stem (hES) cells offers the opportunity both for studying basic developmental processes in vitro as well as for drug screening, modeling diseases, or future cell therapy. Defining protocols for the generation of… Show all

  74. Prof. Dr. Outi Hovatta

    Cultures of human embryonic stem cells: serum replacement medium or serum-containing media and the effect of basic fibroblast growth factor

    20th Feb 2008

    Journal etc.

    PubMed: 15353086

    Abstract:

    Human embryonic stem (hES) cells have traditionally been cultured in medium containing fetal calf serum (FCS) and mouse fibroblasts as feeder cells. The use of animal derived materials carries a risk of transmitting animal pathogens, and they are not… Show all

    Associated cell lines:
  75. Prof. Dr. Outi Hovatta

    Culture and expansion of the human embryonic stem cell line HS 181, evaluated in a double-colour system.

    20th Feb 2008

    Journal etc.

    PubMed: 15345126 DOI: 10.1089/scd.2004.13.337

    Abstract:

    An approach of using RFP-transfected human foreskin fibroblasts (hFS-RFP) to support the growth of GFP expressing human embryonic stem cells (hES; HS181-GFP) is reported. The two-color system was applied to detect interactions between hFS and human e… Show all

    Associated cell lines:
  76. Prof. Dr. Outi Hovatta

    Unique gene expression signature by human embryonic stem cells cultured under serum-free conditions correlates with their enhanced and prolonged growth in an undifferentiated stage

    20th Feb 2008

    Journal etc.

    PubMed: 16100004 DOI: 10.1634/stemcells.2004-0189

    Abstract:

    Understanding the interaction between human embryonic stem cells (hESCs) and their microenvironment is crucial for the propagation and the differentiation of hESCs for therapeutic applications. hESCs maintain their characteristics both in serum-conta… Show all

    Associated cell lines:
  77. Prof. Dr. Outi Hovatta

    Derivation of human embryonic stem cell lines in serum replacement medium using postnatal human fibroblasts as feeder cells.

    20th Feb 2008

    Journal etc.

    PubMed: 15790775 DOI: 10.1634/stemcells.2004-0201

    Abstract:

    Derivation and culture of human embryonic stem cells (hESCs) without animal-derived material would be optimal for cell transplantation. We derived two new hES (HS293 and HS306) and 10 early cell lines using serum replacement (SR) medium instead of co… Show all

    Associated cell lines:
  78. Prof. Dr. Outi Hovatta

    Testing of nine different xeno-free culture media for human embryonic stem cell cultures.

    20th Feb 2008

    Journal etc.

    PubMed: 17251355 DOI: 10.1093/humrep/del523

    Abstract:

    BACKGROUND: Human embryonic stem cells (hESC) are excellent candidates for cell replacement therapies. However, currently used culture conditions contain animal-derived components that bear a risk of transmitting animal pathogens and incorporation of… Show all

  79. Prof. Dr. Outi Hovatta

    Human embryonic stem cells are immunogenic in allogeneic and xenogeneic settings.

    20th Feb 2008

    Journal etc.

    PubMed: 17169186 DOI: 10.1016/j.fertnstert.2005.12.081

    Abstract:

    Recent studies have suggested that human embryonic stem cells (HESC) are immune-privileged and may thereby circumvent rejection. The expression of immunologically active molecules was studied by DNA microarray analysis and by flow cytometry. HESC wer… Show all

  80. Prof. Dr. Outi Hovatta

    Differential lipolytic regulation in human embryonic stem cell-derived adipocytes.

    20th Feb 2008

    Journal etc.

    PubMed: 17426319 DOI: 10.1038/oby.2007.595

    Abstract:

    OBJECTIVE: Human embryonic stem cells (hESCs) have raised great hopes for future clinical applications. Several groups have succeeded in differentiating hESCs into adipocytes, as determined by morphology, mRNA expression, and protein secretion. Howev… Show all

    Associated cell lines:
  81. Prof. Dr. Outi Hovatta

    Distinct sets of developmentally regulated genes that are expressed by human oocytes and human embryonic stem cells.

    20th Feb 2008

    Journal etc.

    PubMed: 17126334 DOI: 10.1016/j.fertnstert.2006.07.1509

    Abstract:

    OBJECTIVE: To identify genes that are expressed differently during final oocyte maturation and early embryonic development in humans. DESIGN: Comparison of gene expression profiles of human germinal vesicle oocytes (hGVO), human embryonic stem cells … Show all

    Associated cell lines:
  82. Prof. Dr. Outi Hovatta

    Expression of leukemia inhibitory factor and its receptors is increased during differentiation of human embryonic stem cells.

    20th Feb 2008

    Journal etc.

    PubMed: 16949591 DOI: 10.1016/j.fertnstert.2005.12.081

    Abstract:

    OBJECTIVE: To investigate gene expression profiles during the early spontaneous differentiation of human embryonic stem cells (hESCs), with particular emphasis on leukemia inhibitory factor (LIF)-induced pathways and the ultrastructural surface morph… Show all

  83. Prof. Dr. Outi Hovatta

    A meta-analysis of human embryonic stem cells transcriptome integrated into a web-based expression atlas.

    20th Feb 2008

    Journal etc.

    PubMed: 17204602 DOI: 10.1634/stemcells.2006-0352

    Abstract:

    Microarray technology provides a unique opportunity to examine gene expression patterns in human embryonic stem cells (hESCs). We performed a meta-analysis of 38 original studies reporting on the transcriptome of hESCs. We determined that 1,076 genes… Show all

    Associated cell lines:
  84. Prof. Dr. Outi Hovatta

    Gene expression signatures of seven individual human embryonic stem cell lines

    20th Feb 2008

    Journal etc.

    PubMed: 16081666 DOI: 10.1634/stemcells.2004-0341

    Abstract:

    Identification of molecular components that define a pluripotent human embryonic stem cell (hESC) provides the basis for understanding the molecular mechanisms regulating the maintenance of pluripotency and induction of differentiation. We compared t… Show all

    Associated cell lines:
  85. Prof. Dr. Outi Hovatta

    A culture system using human foreskin fibroblasts as feeder cells allows production of human embryonic stem cells

    20th Feb 2008

    Journal etc.

    PubMed: 12832363

    Abstract:

    BACKGROUND: Human embryonic stem (hES) cell lines were first cultured using fetal mouse fibroblasts as feeder cells. To avoid feeders and to reduce the amount of xeno-components, Matrigel- and laminin-coated dishes, and conditioned mouse feeder cell … Show all

    Associated cell lines:
  86. Prof. Dr. Marisa Jaconi

    Developmental changes in cardiomyocytes differentiated from human embryonic stem cells: combined molecular and functional approaches.

    16th Jan 2008

    Journal etc.

    PubMed: 17255522 DOI: 10.1634/stemcells.2006-0466

    Abstract:

    Cardiomyocytes derived from human embryonic stem cells constitute a promising cell source for the regeneration of damaged hearts. The assessment of their in vitro functional properties is mandatory to envisage appropriate cardiac cell-based therapies… Show all

    Associated cell lines:
  87. Prof. Dr. Marisa Jaconi

    Fetal bovine serum enables cardiac differentiation of human embryonic stem cells.

    16th Jan 2008

    Journal etc.

    PubMed: 17459089 DOI: 10.1111/j.1432-0436.2007.00174.x

    Abstract:

    During development, cardiac commitment within the mesoderm requires endoderm-secreted factors. Differentiation of embryonic stem cells into the three germ layers in vitro recapitulates developmental processes and can be influenced by supplements adde… Show all

    Associated cell lines:
  88. Dr. Johan Hyllner

    Monitoring differentiation of human embryonic stem cells using real-time PCR

    6th Jan 2008

    Journal etc.

    PubMed: 16081663 DOI: 10.1634/stemcells.2005-0093

    Abstract:

    There is a general lack of rapid, sensitive, and quantitative methods for the detection of differentiating human embryonic stem cells (hESCs). Using light microscopy and immunohistochemistry, we observed that morphological changes of differentiating … Show all

  89. Dr. Johan Hyllner

    Facilitated expansion of human embryonic stem cells by single cell enzymatic dissociation

    6th Jan 2008

    Journal etc.

    PubMed: 17379766 DOI: 10.1634/stemcells.2006-0607

    Abstract:

    Traditionally, human embryonic stem cells (hESCs) are propagated by mechanical dissection or enzymatic dissociation into clusters of cells. To facilitate up-scaling and the use of hESC in various experimental manipulations, such as fluorescence-activ… Show all

  90. Dr. Johan Hyllner

    Large-scale propagation of four undifferentiated human embryonic stem cell lines in a feeder-free culture system

    6th Jan 2008

    Journal etc.

    PubMed: 15965986 DOI: 10.1002/dvdy.20459

    Abstract:

    We describe an improved and more robust protocol for transfer and subsequent propagation of human embryonic stem cells under feeder-free conditions. The results show that mechanical dissociation for transfer of the human embryonic stem cells to Matri… Show all

  91. Dr. Johan Hyllner

    Artifactual insulin release from differentiated embryonic stem cells

    6th Jan 2008

    Journal etc.

    PubMed: 15448090

    Abstract:

    Several recent reports claim the generation of insulin-producing cells from embryonic stem cells via the differentiation of progenitors that express nestin. Here, we investigate further the properties of these insulin-containing cells. We find that a… Show all

    Associated cell lines:
  92. Dr. Johan Hyllner

    Genomic alterations in cultured human embryonic stem cells

    6th Jan 2008

    Journal etc.

    PubMed: 16142235 DOI: 10.1038/ng1631

    Abstract:

    Cultured human embryonic stem cell (hESC) lines are an invaluable resource because they provide a uniform and stable genetic system for functional analyses and therapeutic applications. Nevertheless, these dividing cells, like other cells, probably u… Show all

    Associated cell lines:
  93. Dr. Johan Hyllner

    Expression of drug metabolizing enzymes in hepatocyte-like cells derived from human embryonic stem cells

    6th Jan 2008

    Journal etc.

    PubMed: 17568565 DOI: 10.1016/j.bcp.2007.05.009

    Abstract:

    Human embryonic stem cells (hESC) offer a potential unlimited source for functional human hepatocytes, since they can differentiate into hepatocyte-like cells displaying a characteristic hepatic morphology and expressing several hepatic markers. Such… Show all

    Associated cell lines:
  94. Dr. Johan Hyllner

    Glutathione transferases in hepatocyte-like cells derived from human embryonic stem cells

    6th Jan 2008

    Journal etc.

    PubMed: 17346923 DOI: 10.1016/j.tiv.2007.01.021

    Abstract:

    Human embryonic stem cells (hESCs) offer a potential unlimited source for functional human hepatocytes, since hESCs can differentiate into hepatocyte-like cells displaying a characteristic hepatic morphology and expressing several hepatic markers. Th… Show all

  95. Dr. Johan Hyllner

    Chromosomal integrity maintained in five human embryonic stem cell lines after prolonged in vitro culture

    4th Jan 2008

    Journal etc.

    PubMed: 16544187 DOI: 10.1007/s10577-006-1019-8

    Abstract:

    There have been recent reports of human embryonic stem cell (hESC) lines developing chromosomal aberrations after long-term culture, indicating an unstable genomic status due to the in vitro milieu. This raises concern, since it would limit their use… Show all

  96. Dr. Johan Hyllner

    Derivation of a xeno-free human embryonic stem cell line

    4th Jan 2008

    Journal etc.

    PubMed: 16741223 DOI: 10.1634/stemcells.2006-0130

    Abstract:

    Elimination of all animal material during both the derivation and long-term culture of human embryonic stem cells (hESCs) is necessary prior to future application of hESCs in clinical cell therapy. The potential consequences of transplanting xeno-con… Show all

    Associated cell lines:
  97. Dr. Johan Hyllner

    High-resolution analysis of the subtelomeric regions of human embryonic stem cells

    4th Jan 2008

    Journal etc.

    PubMed: 15790769 DOI: 10.1634/stemcells.2004-0254

    Abstract:

    The use of human embryonic stem cells (hESCs) in most applications is dependent on their undifferentiated proliferation in vitro. Recent studies have illustrated the possibility that chromosomal changes may occur in hESCs during in vitro propagation … Show all

  98. Dr. Johan Hyllner

    Differentiating human embryonic stem cells express a unique housekeeping gene signature

    4th Jan 2008

    Journal etc.

    PubMed: 17284652 DOI: 10.1634/stemcells.2006-0247

    Abstract:

    Housekeeping genes (HKGs) are involved in basic functions needed for the sustenance of the cell and are assumed to be constitutively expressed at a constant level. Based on these features, HKGs are frequently used for normalization of gene expression… Show all

    Associated cell lines:
  99. Dr. Johan Hyllner

    Human embryonic stem cells have a unique epigentic signature

    4th Jan 2008

    Journal etc.

    Associated cell lines:
  100. Dr. Johan Hyllner

    Derivation, characterization and differentiation of human embryonic stem cells

    4th Jan 2008

    Journal etc.

    PubMed: 15153613 DOI: 10.1634/stemcells.22-3-367

    Abstract:

    The derivation of human embryonic stem (hES) cells establishes a new avenue to approach many issues in human biology and medicine for the first time. To meet the increased demand for characterized hES cell lines, we present the derivation and charact… Show all

  101. Dr. Johan Hyllner

    Clonal derivation and characterization of human embryonic stem cell lines

    4th Jan 2008

    Journal etc.

    PubMed: 16324761 DOI: 10.1016/j.jbiotec.2005.10.010

    Abstract:

    Human embryonic stem cells (hESC) are isolated as clusters of cells from the inner cell mass of blastocysts and thus should formally be considered as heterogeneous cell populations. Homogenous hESC cultures can be obtained through subcloning. Here, w… Show all

    Associated cell lines:
  102. Dr. Johan Hyllner

    Molecular and pharmacological properties of human embryonic stem cell-derived cardiomyocytes

    4th Jan 2008

    Journal etc.

    PubMed: 17138763

    Abstract:

    Human embryonic stem cells (hESCs) can be coaxed to differentiate into specific cell types, including cardiomyocyte-like cells. These cells express cardiac-specific markers and display functional similarities to their adult counterparts. Based on the… Show all

    Associated cell lines:
  103. Prof. Dr. Douglas A. Melton

    Derivation of Embryonic Stem-cell Lines from Human Blastocysts

    20th Dec 2007

    Journal etc.

    PubMed: 14999088 DOI: 10.1056/NEJMsr040330

    Abstract:

    Embryonic stem cells have the unique ability to form all adult cell types. Harnessing this potential may provide a source of cells to replace those that are lost or impaired as a result of disease. Moreover, the derivation of human embryonic … Show all

  104. Prof. Dr, Karen Sermon

    Derivation of human embryonic stem cell lines from embryos obtained after IVF and after PGD for monogenic disorders.

    11th Dec 2007

    Journal etc.

    PubMed: 16284066 DOI: 10.1093/humrep/dei345

    Abstract:

    BACKGROUND: Human embryonic stem (hES) cells are pluripotent cells usually derived from the inner cell mass (ICM) of blastocysts. Because of their ability to differentiate into all three embryonic germ layers, hES cells represent an important materia… Show all

  105. Dr. Claus Yding Andersen

    Regional differences in expression of specific markers for human embryonic stem cells

    4th Dec 2007

    Journal etc.

    PubMed: 17623545

    Abstract:

    Characterization of human embryonic stem cell (hESC) lines derived from the inner cell masses of blastocysts generally includes expression analysis of markers such as OCT4, NANOG, SSEA3, SSEA4, TRA-1-60 and TRA-1-81. Expression is usually detected by… Show all