DS4, UWWC1-DS4

General

Cell Line

hPSCreg name WISCi005-B
Cite as:
WISCi005-B (RRID:CVCL_EJ83)
Alternative name(s)
DS4, UWWC1-DS4
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
WISCi005-A
(DS1, UWWC1-DS1)
Donor diseases:
Trisomy 21
WISCi005-C
(UWWC1-DS2U, DS2U)
Donor diseases:
Trisomy 21
UPSFRi005-A-1
(PB06-1)
Donor diseases:
Down syndrome
NCHi004-A
(NCH140)
Donor diseases:
Down Syndrome
SCVIi085-A
Donor diseases:
Down syndrome
SCVIi086-A
Donor diseases:
Down syndrome
WISCi006-A
(2DS3, UWWC1-2DS3)
Donor diseases:
Down syndrome
NCHi010-A
(NCH141)
Donor diseases:
Down syndrome
UPSFRi005-A
(PB06)
Donor diseases:
Down syndrome
NSi003-A
(NSi-DS)
Donor diseases:
Down Syndrome
NSi003-B
(NSi-isoEu)
Donor diseases:
Down Syndrome
Last update 22nd May 2019
Notes The fibroblasts from Coriell turned out to be mosaic (some trisomy, some (10%) normal). Thus, we were able to generate both control and trisomy 21 iPSC lines.
User feedback
No feedback available yet.

Login to share your feedback, experiences or results with the research community.

Provider

Generator University of Wisconsin (WISC)
Contact:
Waisman Center
Distributors

External Databases

BioSamples SAMEA104388691
Cellosaurus CVCL_EJ83
Wikidata Q54994098

General Information

Publications
Projects
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: allowed
Commercial use: allowed

Donor Information

General Donor Information

Sex male
Age of donor (at collection) 1-4

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Synonyms
  • Trisomy 21
  • Trisomy-21

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
Karyotyping method: G-Banding

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA104242073

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Please provide contact information of the holder of the original Donor Information Sheet. Coriell Institute for Medical Research, https://www.coriell.org/
Do you (Depositor/Provider) hold the original Donor Consent Form? No
If you do not hold the Donor Consent Form, do you know who does? No
Alternatives to consent
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent expressly prevent the derivation of pluripotent stem cells? No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
How may genetic information associated with the cell line be accessed? Open Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Coriell Institute for Medical Research, https://www.coriell.org/
Approval number
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? Yes
Name of accrediting authority involved? Coriell Institute for Medical Research, https://www.coriell.org/
Approval number
Is there an MTA available for the cell line? Yes
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell type
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.; These cells may be vimentin-positive, fibronectin-positive, fsp1-positive, MMP-1-positive, collagen I-positive, collagen III-positive, and alpha-SMA-negative.
Age of donor (at collection) 1-4

Reprogramming method

Vector type Integrating
Vector Virus (Retrovirus)
Genes
Is the used vector excisable?
Unknown
Absence of reprogramming vector(s)?
Unknown
Reprogramming vectors silenced?

Vector free reprogramming

Other

Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzymatically
Medium mTeSR™ 1
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
Unknown
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Unknown

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
SOX2
Yes
SSEA-4
Yes
TRA 1-81
Yes
Method documentation
Differentiation Potency
Endoderm
Ont Id: NCRO_0000112
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation
Neuron
Ont Id: CL_0000540
Neuroepithelial Stem Cell
Ont Id: CL_0002259

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
47,XY,+21
Karyotyping method: G-Banding

Other Genotyping (Cell Line)