2DS3, UWWC1-2DS3

General#

Cell Line

hPSCreg Name WISCi006-A
Alternative name(s)
2DS3, UWWC1-2DS3
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 8th December 2017
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Provider

Generator University of Wisconsin (WISC)
Contact:
Waisman Center
Owner Madison
Distributors

External Databases

BioSamples SAMEA104381150
Cellosaurus CVCL_EJ80

General Information

Publications
Is the cell line available in principle? Available, without restrictions

Donor Information#

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Down syndrome
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • DOWN'S SYNDROME
  • Trisomy 21 NOS
  • Trisomy 21, Mitotic Nondisjunction
  • Trisomy 21, Meiotic Nondisjunction
  • Down's syndrome NOS (disorder)
  • Complete trisomy 21 syndrome (disorder)
  • G Trisomy
  • Down's syndrome NOS
  • Partial Trisomy 21 Down Syndrome
  • Down Syndrome, Partial Trisomy 21
  • Syndrome, Down's
  • Trisomy 21
  • T21 - Trisomy 21
  • Syndrome, Down
  • Complete trisomy 21 syndrome
  • trisomy 21 syndrome
  • Mongolism
  • Downs Syndrome
  • Trisomy 21 (Down Syndrome)Downs SyndromeTrisomy 21 Syndrome
  • Down's syndrome - trisomy 21
show more synonyms

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
47,XY,+21
Karyotyping method: G-Banding

External Databases (Donor)

BioSamples SAMEA104381151

Ethics#

We are currently revising this section.

hIPSC Derivation#

General

Source cell type
fibroblast
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.
Age of donor (at collection) neonate

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Is reprogramming vector detectable?
No
Methods used
PCR, RT-PCR
Notes on reprogramming vector detection Fig S1
Files and images showing reprogramming vector expressed or silenced

Vector free reprogramming

Other

Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Surface coating Matrigel/Geltrex
Feeder cells
No
Medium mTeSR™ 1
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SOX2
Yes
SSEA-4
Yes
TRA 1-81
Yes
teratoma foration in SCID mice Fig S1 Weick et al., 2013
Differentiation Potency

Certificate of Analysis

Is there a certificate of analysis available?
Yes
Passage:

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
47,XY,+21
Karyotyping method: G-Banding

Other Genotyping (Cell Line)