2DS3, UWWC1-2DS3

General

Cell Line

hPSCreg name WISCi006-A
Cite as:
WISCi006-A (RRID:CVCL_EJ80)
Alternative name(s)
2DS3, UWWC1-2DS3
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
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(PB06-1)
Donor diseases:
Down syndrome
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SCVIi086-A
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WISCi005-A
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Donor diseases:
Trisomy 21
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Trisomy 21
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(UWWC1-DS2U, DS2U)
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Trisomy 21
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Last update 22nd May 2019
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Provider

Generator University of Wisconsin (WISC)
Contact:
Waisman Center
Owner Madison
Distributors

External Databases

BioSamples SAMEA104381150
Cellosaurus CVCL_EJ80
Wikidata Q54994101

General Information

Publications
Projects
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: allowed
Commercial use: allowed

Donor Information

General Donor Information

Sex male
Age of donor (at collection) neonate

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • DOWN'S SYNDROME
  • Trisomy 21 NOS
  • Trisomy 21, Mitotic Nondisjunction
  • Trisomy 21, Meiotic Nondisjunction
  • Down's syndrome NOS (disorder)
  • Complete trisomy 21 syndrome (disorder)
  • G Trisomy
  • Down's syndrome NOS
  • Partial Trisomy 21 Down Syndrome
  • Down Syndrome, Partial Trisomy 21
  • Syndrome, Down's
  • T21 - Trisomy 21
  • Syndrome, Down
  • trisomy 21 syndrome
  • Mongolism
  • Downs Syndrome
  • Trisomy 21 (Down Syndrome)Downs SyndromeTrisomy 21 Syndrome
  • Down's syndrome - trisomy 21
  • Downs syndrome
  • trisomy 21 (Down syndrome)
  • Down's syndrome
  • G trisomy
  • Down syndrome
  • Complete trisomy 21 syndrome
  • Down syndrome, Isolated cases
  • leukemia, megakaryoblastic, with or without Down syndrome, somatic
  • Trisomy 21
  • complete trisomy 21 syndrome
  • trisomy 21
  • Down syndrome chromosome region
  • Down syndrome critical region
  • leukemia, megakaryoblastic, of Down syndrome
  • transient myeloproliferative disorder of Down syndrome
show more synonyms

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
47,XY,+21
Karyotyping method: G-Banding

External Databases (Donor)

BioSamples SAMEA104381151

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Please provide contact information of the holder of the original Donor Information Sheet. https://catalog.coriell.org/0/Sections/Search/Sample_Detail.aspx?Ref=GM02504&Product=CC
Do you (Depositor/Provider) hold the original Donor Consent Form? No
If you do not hold the Donor Consent Form, do you know who does? Yes
Please provide the contact information https://catalog.coriell.org/; Coriell Biorepositories and Services
Alternatives to consent
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent expressly prevent the derivation of pluripotent stem cells? No
Does consent pertain to a specific research project? No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? Yes

Does consent permit research by

an academic institution? Yes
a non-profit company? Yes
a for-profit corporation? Yes
How may genetic information associated with the cell line be accessed? Open Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Does the consent anticipate that the donor will be notified of results or outcomes of any research involving the donated samples or derived cells? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? https://catalog.coriell.org/; oriell Biorepositories and Services
Approval number
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? Yes
Name of accrediting authority involved? Coriell Biorepositories and Services
Approval number
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell line name GM02504
Source cell type
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.; These cells may be vimentin-positive, fibronectin-positive, fsp1-positive, MMP-1-positive, collagen I-positive, collagen III-positive, and alpha-SMA-negative.
Age of donor (at collection) neonate
Source cell line vendor Coriell

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Is reprogramming vector detectable?
No
Methods used
PCR, RT-PCR
Notes on reprogramming vector detection Fig S1
Files and images showing reprogramming vector expressed or silenced

Vector free reprogramming

Other

Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Medium mTeSR™ 1
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SOX2
Yes
SSEA-4
Yes
TRA 1-81
Yes
teratoma foration in SCID mice Fig S1 Weick et al., 2013
Differentiation Potency

Certificate of Analysis

Is there a certificate of analysis available?
Yes
Passage:

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
47,XY,+21
Karyotyping method: G-Banding

Other Genotyping (Cell Line)