NSi-isoEu

The cell line is not validated yet.

General

Cell Line

hPSCreg Name
NSi003-B
Alternative name(s)
NSi-isoEu
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 1st February 2023
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Provider

Generator Stem Cell and Neurobiology Lab (NS)

External Databases

BioSamples SAMEA112442037

General Information

* Is the cell line readily obtainable for third parties?
No

Donor Information

General Donor Information

Sex male
Ethnicity Caucasian

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Down Syndrome
The donor is affected.
Synonyms
  • Down Syndrome
  • Trisomy 21
  • Down's Syndrome
  • Trisomy 21 Syndrome
  • Down syndrome
  • Trisomy 21 (Down Syndrome)
show more synonyms

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
Trisomy (47;XY)
Karyotyping method: G-Banding

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA112442036

Ethics

Do you (Depositor/Provider) hold the original Donor Consent Form? No
If you do not hold the Donor Consent Form, do you know who does? No
Alternatives to consent are available? Yes
Alternatives to consent ICSCR approval hiPSCs
Alternative consent approval number
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
How may genetic information associated with the cell line be accessed? No information
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? IC-SCR Committee of NCCS
Approval number (NCCS/IC-SCR/2021-I/6)
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell line name Detroit-532 Fibroblasts (CCL-54™ from ATCC)
Source cell type
Fibroblast
Synonyms
  • fibroblast
  • Fibroblasts
  • Fibroblast
  • FIBROBLAST
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Source cell origin
Skin
An organ that constitutes the external surface of the body. It consists of the epidermis, dermis, and skin appendages.
Synonyms
  • Skin
  • Human Skin
  • SKIN
  • Integument
  • Skin, total
  • Skin, NOS
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Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Is reprogramming vector detectable?
Yes
Methods used
RT-PCR
Files and images showing reprogramming vector expressed or silenced

Vector free reprogramming

Type of used vector free reprogramming factor(s)
None

Other

Selection criteria for clones Trisomic hiPSCs were cultured for many passages to lose an extra copy of chromosome 21. After several passages, a single clonal selection was performed to get a disomic clone which was confirmed through karyotyping.
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions

Surface coating CF1 feeders
Feeder cells Mouse embryonic fibroblasts
Passage method Enzymatically
Collagenase
CO2 Concentration 5 %
Medium Other medium:
Base medium: KO-DMEM-F12
Main protein source: Knock-out serum replacement
Serum concentration: 20 %
Supplements
Basic fibroblast growth factor 25 ng/ml
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SOX2
Yes
NANOG
Yes
TRA 1-60
Yes
TRA 1-81
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Hepatocyte
Ont Id: CL_0000182
In vitro directed differentiation
Mesoderm
Ont Id: UBERON_0000926
In vitro directed differentiation
Smooth Muscle Cell
Ont Id: CL_0000192
In vitro directed differentiation
Ectoderm
Ont Id: UBERON_0000924
In vitro directed differentiation
Neuron
Ont Id: CL_0000540
In vitro directed differentiation

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
Disomy (46;XY)
Karyotyping method: G-Banding

Other Genotyping (Cell Line)