NSi-isoEu
The cell line is not validated yet.
NSi003-B
General
Cell Line |
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| hPSCreg name | NSi003-B |
| Cite as: | NSi003-B (RRID:CVCL_D0KB) |
| Alternative name(s) |
NSi-isoEu
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| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines |
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| Last update | 1st February 2023 |
| User feedback | |
Provider |
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| Generator | Stem Cell and Neurobiology Lab (NS) |
External Databases |
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| BioSamples | SAMEA112442037 |
| Cellosaurus | CVCL_D0KB |
| Wikidata | Q123033201 |
General Information |
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| Publications | |
| * Is the cell line readily obtainable for third parties? |
No |
Donor Information
General Donor Information |
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| Sex | male |
| Ethnicity | Caucasian |
Phenotype and Disease related information (Donor) |
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| Diseases | A disease was diagnosed.
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Karyotyping (Donor) |
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| Has the donor karyotype been analysed? |
Yes
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Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
No
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Donor Relations |
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| Other cell lines of this donor | |
External Databases (Donor) |
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| BioSamples | SAMEA112442036 |
Ethics
| Do you (Depositor/Provider) hold the original Donor Consent Form? | No |
| If you do not hold the Donor Consent Form, do you know who does? | No |
| Alternatives to consent are available? | Yes |
| Alternatives to consent | ICSCR approval hiPSCs |
| Alternative consent approval number | |
| Does consent explicitly allow the derivation of pluripotent stem cells? | Yes |
| How may genetic information associated with the cell line be accessed? | No information |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | Yes |
| Name of accrediting authority involved? | IC-SCR Committee of NCCS |
| Approval number | (NCCS/IC-SCR/2021-I/6) |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? |
hIPSC Derivation
General |
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| Source cell line name | Detroit-532 Fibroblasts (CCL-54™ from ATCC) |
| Source cell type |
Synonyms
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| Source cell origin |
An organ that constitutes the external surface of the body. It consists of the epidermis, dermis, and skin appendages.
Synonyms
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Reprogramming method |
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| Vector type | Non-integrating |
| Vector | Sendai virus |
| Is reprogramming vector detectable? |
Yes |
| Methods used |
RT-PCR
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| Files and images showing reprogramming vector expressed or silenced | |
Vector free reprogramming |
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| Type of used vector free reprogramming factor(s) |
None
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Other |
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| Selection criteria for clones | Trisomic hiPSCs were cultured for many passages to lose an extra copy of chromosome 21. After several passages, a single clonal selection was performed to get a disomic clone which was confirmed through karyotyping. |
| Derived under xeno-free conditions |
No |
| Derived under GMP? |
No |
| Available as clinical grade? |
No |
Culture Conditions
| Surface coating | CF1 feeders | ||||||
| Feeder cells |
Mouse embryonic fibroblasts |
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| Passage method |
Enzymatically
Collagenase
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| CO2 Concentration | 5 % | ||||||
| Medium |
Other medium:
Base medium: KO-DMEM-F12
Main protein source: Knock-out serum replacement Serum concentration: 20 % Supplements
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| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
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| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | Yes |
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| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| POU5F1 (OCT-4) |
Yes |
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| SOX2 |
Yes |
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| NANOG |
Yes |
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| TRA 1-60 |
Yes |
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| TRA 1-81 |
Yes |
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Differentiation Potency
Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
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Other Genotyping (Cell Line) |
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