General

Cell Line

hPSCreg name SCVIi086-A
Cite as:
SCVIi086-A (RRID:CVCL_D0L6)
Cell line type Human induced pluripotent stem cell (hiPSC)
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Last update 24th August 2023
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Provider

Generator Stanford Cardiovascular Institute (SCVI)
Owner Stanford Cardiovascular Institute (SCVI)
Distributors

External Databases

BioSamples SAMEA114242735
Cellosaurus CVCL_D0L6
Wikidata Q123033428

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: allowed
Commercial use: not allowed

Donor Information

General Donor Information

Sex male
Ethnicity White

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • DOWN'S SYNDROME
  • Trisomy 21 NOS
  • Trisomy 21, Mitotic Nondisjunction
  • Trisomy 21, Meiotic Nondisjunction
  • Down's syndrome NOS (disorder)
  • Complete trisomy 21 syndrome (disorder)
  • G Trisomy
  • Down's syndrome NOS
  • Partial Trisomy 21 Down Syndrome
  • Down Syndrome, Partial Trisomy 21
  • Syndrome, Down's
  • T21 - Trisomy 21
  • Syndrome, Down
  • trisomy 21 syndrome
  • Mongolism
  • Downs Syndrome
  • Trisomy 21 (Down Syndrome)Downs SyndromeTrisomy 21 Syndrome
  • Down's syndrome - trisomy 21
  • Downs syndrome
  • trisomy 21 (Down syndrome)
  • Down's syndrome
  • G trisomy
  • Down syndrome
  • Complete trisomy 21 syndrome
  • Down syndrome, Isolated cases
  • leukemia, megakaryoblastic, with or without Down syndrome, somatic
  • Trisomy 21
  • complete trisomy 21 syndrome
  • trisomy 21
  • Down syndrome chromosome region
  • Down syndrome critical region
  • leukemia, megakaryoblastic, of Down syndrome
  • transient myeloproliferative disorder of Down syndrome
show more synonyms

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
Trisomy 21
Karyotyping method: Molecular karyotyping by SNP array
http://

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA114242736

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Please provide contact information of the holder of the original Donor Information Sheet. joewu@stanford.edu
Do you (Depositor/Provider) hold the original Donor Consent Form? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
How may genetic information associated with the cell line be accessed? Controlled Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? No
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell type
A peripheral blood cell with a single nucleus. This category includes lymphocytes and monocytes.
Synonyms
  • PERIPHERAL BLOOD MONONUCLEAR CELL
  • Peripheral Blood Mononuclear Cell
  • PBMC
Source cell origin
A liquid tissue; its major function is to transport oxygen throughout the body. It also supplies the tissues with nutrients, removes waste products, and contains various components of the immune system defending the body against infection. Several hormones also travel in the blood.
Synonyms
  • Reticuloendothelial System, Blood
  • Peripheral Blood
  • peripheral blood
  • blood
  • Blood
  • Whole Blood
  • BLOOD
  • portion of blood
  • vertebrate blood
show more synonyms

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Is reprogramming vector detectable?
No
Notes on reprogramming vector detection RT-PCR

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Surface coating Matrigel/Geltrex
Passage method Enzyme-free cell dissociation
EDTA
CO2 Concentration 5 %
Medium Other medium:
Base medium: StemMACS iPS-Brew XF
Main protein source:
Serum concentration: %
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
SOX2
Yes
NANOG
Yes
POU5F1 (OCT-4)
Yes
Morphology pictures
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
Marker Expressed
SOX17
Yes
FOXA2
Yes
Mesoderm
Ont Id: UBERON_0000926
Marker Expressed
Brachyury
Yes
TBX6
Yes
Ectoderm
Ont Id: UBERON_0000924
Marker Expressed
PAX6
Yes
OTX2
Yes

Microbiology / Virus Screening

Mycoplasma Negative

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
Trisomy 21
Passage number: 27
Karyotyping method: Molecular karyotyping by SNP array
http://

Other Genotyping (Cell Line)