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SCVIi085-A

Registration Summary:
SCVIi085-A

General

Cell Line
hPSCreg name SCVIi085-A
Cite as:
SCVIi085-A (RRID:CVCL_D0L5)
Cell line type Human induced pluripotent stem cell (hiPSC)
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Last update 23rd August 2023
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Provider
Generator Stanford Cardiovascular Institute (SCVI)
Owner Stanford Cardiovascular Institute (SCVI)
Distributors
Derivation country United States

External Databases
BioSamples SAMEA114242733
Cellosaurus CVCL_D0L5
Wikidata Q123033427

General Information
Publications
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: allowed
Commercial use: not allowed

Donor Information

General Donor Information
Sex male
Ethnicity South Asian

Phenotype and Disease related information (Donor)
Diseases A disease was diagnosed.
Down syndrome
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • DOWN'S SYNDROME
  • Trisomy 21 NOS
  • Trisomy 21, Mitotic Nondisjunction
  • Trisomy 21, Meiotic Nondisjunction
  • Down's syndrome NOS (disorder)
  • Complete trisomy 21 syndrome (disorder)
  • G Trisomy
  • Down's syndrome NOS
  • Partial Trisomy 21 Down Syndrome
  • Down Syndrome, Partial Trisomy 21
  • Syndrome, Down's
  • T21 - Trisomy 21
  • Syndrome, Down
  • trisomy 21 syndrome
  • Mongolism
  • Downs Syndrome
  • Trisomy 21 (Down Syndrome)Downs SyndromeTrisomy 21 Syndrome
  • Down's syndrome - trisomy 21
  • Downs syndrome
  • trisomy 21 (Down syndrome)
  • Down's syndrome
  • G trisomy
  • Down syndrome
  • Complete trisomy 21 syndrome
  • Down syndrome, Isolated cases
  • leukemia, megakaryoblastic, with or without Down syndrome, somatic
  • Trisomy 21
  • complete trisomy 21 syndrome
  • trisomy 21
  • Down syndrome chromosome region
  • Down syndrome critical region
  • leukemia, megakaryoblastic, of Down syndrome
  • transient myeloproliferative disorder of Down syndrome
show more synonyms

Karyotyping (Donor)
Has the donor karyotype been analysed?
Yes
Trisomy 21
Karyotyping method: Molecular karyotyping by SNP array
http://

Other Genotyping (Donor)
Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)
BioSamples SAMEA114242734

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Provide contact information of the holder of the original Donor Information Sheet: joewu@stanford.edu
Do you (Depositor/Provider) hold the original Donor Consent Form? No
If you do not hold the Donor Consent Form, do you know who does? Yes
Contact information / weblink joewu@stanford.edu
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No

Does consent permit research by

an academic institution? Yes
How may genetic information associated with the cell line be accessed? Controlled Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? No
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General
Source cell type
Peripheral Blood Mononuclear Cell
A peripheral blood cell with a single nucleus. This category includes lymphocytes and monocytes.
Synonyms
  • PERIPHERAL BLOOD MONONUCLEAR CELL
  • Peripheral Blood Mononuclear Cell
  • PBMC

Reprogramming method
Vector type Non-integrating
Vector Sendai virus

Vector free reprogramming

Other
Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Surface coating Matrigel/Geltrex
Passage method Enzyme-free cell dissociation
EDTA
CO2 Concentration 5 %
Medium Other medium:
Base medium: StemMACS iPS-Brew XF
Main protein source:
Serum concentration: %

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
SOX2
Yes
–
NANOG
Yes
–
–
POU5F1 (OCT-4)
Yes
–
Score:
Marker Present Absent
mCpG
OCT4
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Marker Expressed
SOX17
Yes
FOXA2
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro directed differentiation
Marker Expressed
TBX6
Yes
Brachyury
Yes
Ectoderm
Ont Id: UBERON_0000924
In vitro directed differentiation
Marker Expressed
OTX2
Yes
PAX6
Yes

Microbiology / Virus Screening
Mycoplasma Negative

Genotyping

Karyotyping (Cell Line)
Has the cell line karyotype been analysed?
Yes
Trisomy 21
Karyotyping method: Molecular karyotyping by SNP array
http://

Other Genotyping (Cell Line)