UPSFRi005-A

PB06

General

Cell Line

hPSCreg name UPSFRi005-A
Cite as:
UPSFRi005-A (RRID:CVCL_C349)
Alternative name(s)
PB06
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
UPSFRi005-A-1
(PB06-1)
Donor diseases:
Down syndrome
NCHi004-A
(NCH140)
Donor diseases:
Down Syndrome
SCVIi085-A
Donor diseases:
Down syndrome
SCVIi086-A
Donor diseases:
Down syndrome
WISCi005-A
(DS1, UWWC1-DS1)
Donor diseases:
Trisomy 21
WISCi006-A
(2DS3, UWWC1-2DS3)
Donor diseases:
Down syndrome
WISCi005-B
(DS4, UWWC1-DS4)
Donor diseases:
Trisomy 21
WISCi005-C
(UWWC1-DS2U, DS2U)
Donor diseases:
Trisomy 21
NCHi010-A
(NCH141)
Donor diseases:
Down syndrome
NSi003-A
(NSi-DS)
Donor diseases:
Down Syndrome
NSi003-B
(NSi-isoEu)
Donor diseases:
Down Syndrome
Last update 31st August 2022
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Provider

Generator Université Paris-Sud 11 (UPSFR)
Contact:
ESTeam Paris Sud
Derivation country France

External Databases

Cellosaurus CVCL_C349
Wikidata Q54991770

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Subclones

Donor Information

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
The donor is affected.
Synonyms
  • DOWN'S SYNDROME
  • Trisomy 21 NOS
  • Trisomy 21, Mitotic Nondisjunction
  • Trisomy 21, Meiotic Nondisjunction
  • Down's syndrome NOS (disorder)
  • Complete trisomy 21 syndrome (disorder)
  • G Trisomy
  • Down's syndrome NOS
  • Partial Trisomy 21 Down Syndrome
  • Down Syndrome, Partial Trisomy 21
  • Syndrome, Down's
  • T21 - Trisomy 21
  • Syndrome, Down
  • trisomy 21 syndrome
  • Mongolism
  • Downs Syndrome
  • Trisomy 21 (Down Syndrome)Downs SyndromeTrisomy 21 Syndrome
  • Down's syndrome - trisomy 21
  • Downs syndrome
  • trisomy 21 (Down syndrome)
  • Down's syndrome
  • G trisomy
  • Down syndrome
  • Complete trisomy 21 syndrome
  • Down syndrome, Isolated cases
  • leukemia, megakaryoblastic, with or without Down syndrome, somatic
  • Trisomy 21
  • complete trisomy 21 syndrome
  • trisomy 21
  • Down syndrome chromosome region
  • Down syndrome critical region
  • leukemia, megakaryoblastic, of Down syndrome
  • transient myeloproliferative disorder of Down syndrome
show more synonyms

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Provide contact information of the holder of the original Donor Information Sheet (publicly visible) :
Do you (Depositor/Provider) hold the original Donor Consent Form? No
If you do not hold the Donor Consent Form, do you know who does? No
How may genetic information associated with the cell line be accessed?
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell type
A cell of a fetus which is suspended in the amniotic fluid. Amniocytes are considered to arise from several tissues including fetal skin, the fetal urinary tract, umbilical cord, and the inner amniotic surface.

Reprogramming method

Vector type Integrating
Vector Virus (Lentivirus)
Genes
Is the used vector excisable?
Unknown
Absence of reprogramming vector(s)?
Unknown
Reprogramming vectors silenced?

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Feeder cells mouse embryonic fibroblast cell
Cellfinder Ont Id: EFO_0004040
CO2 Concentration 5 %
Medium Other medium:
Base medium: DMEM/F-12
Main protein source: Knock-out serum replacement
Serum concentration: 20 %
Supplements
bFGF
2-mercaptoethanol

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
POU5F1 (OCT-4)
Yes
SSEA-3
Yes
SSEA-4
Yes
TRA 1-60
Yes
HESCA-1
Yes

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
47XY, +21

Other Genotyping (Cell Line)