NSi-DS

The cell line is not validated yet.

General

Cell Line

hPSCreg name NSi003-A
Cite as:
NSi003-A (RRID:CVCL_D0KA)
Alternative name(s)
NSi-DS
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines No similar lines found.
Last update 1st February 2023
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Provider

Generator Stem Cell and Neurobiology Lab (NS)
Derivation country India

External Databases

BioSamples SAMEA112442035
Cellosaurus CVCL_D0KA
Wikidata Q123033200

General Information

Publications
* Is the cell line readily obtainable for third parties?
No

Donor Information

General Donor Information

Sex male
Ethnicity Caucasian

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
The donor is affected.
Synonyms
  • Down Syndrome
  • Trisomy 21
  • Down's Syndrome
  • Trisomy 21 Syndrome
  • Down syndrome
  • Trisomy 21 (Down Syndrome)
show more synonyms

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
Trisomy 21(47;XY)
Karyotyping method: G-Banding

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA112442036

Ethics

Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Provide contact information of the holder of the original Donor Information Sheet: Detroit-532 Fibroblasts (CCL-54™ from ATCC)
Do you (Depositor/Provider) hold the original Donor Consent Form? No
If you do not hold the Donor Consent Form, do you know who does? No
Alternatives to consent are available? Yes
Alternatives to consent ICSCR approval hiPSCs
Alternative consent approval number
Has the donor agreed to be re-contacted? Unknown
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
How may genetic information associated with the cell line be accessed? No information
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? IC-SCR Committee of NCCS
Approval number (NCCS/IC-SCR/2021-I/6)
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell line name Detroit-532 Fibroblasts (CCL-54™ from ATCC)
Source cell type
Synonyms
  • fibroblast
  • Fibroblasts
  • Fibroblast
  • FIBROBLAST
show more synonyms
Source cell origin
An organ that constitutes the external surface of the body. It consists of the epidermis, dermis, and skin appendages.
Synonyms
  • Skin
  • Human Skin
  • SKIN
  • Integument
  • Skin, total
  • Skin, NOS
  • Skin structure (body structure)
  • entire skin
  • skin of body
  • skin organ
show more synonyms

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Is reprogramming vector detectable?
Yes
Methods used
RT-PCR
Files and images showing reprogramming vector expressed or silenced

Vector free reprogramming

Type of used vector free reprogramming factor(s)
None

Other

Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions

Surface coating CF1 feeders
Feeder cells mouse embryonic fibroblasts
Passage method Enzymatically
Collagenase
CO2 Concentration 5 %
Medium Other medium:
Base medium: KO-DMEM-F12
Main protein source: Knock-out serum replacement
Serum concentration: 20 %
Supplements
Basic fibroblast growth factor 25 ng/ml
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SOX2
Yes
NANOG
Yes
TRA 1-60
Yes
TRA 1-81
Yes
Marker Present Absent
mCpG
OCT4
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Hepatocyte
Ont Id: CL_0000182
In vitro directed differentiation
Mesoderm
Ont Id: UBERON_0000926
In vitro directed differentiation
Smooth Muscle Cell
Ont Id: CL_0000192
In vitro directed differentiation
Ectoderm
Ont Id: UBERON_0000924
In vitro directed differentiation
Neuron
Ont Id: CL_0000540
In vitro directed differentiation

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
Trisomy 21(47;XY)
Karyotyping method: G-Banding

Other Genotyping (Cell Line)